Article

Effect of low-level laser irradiation on in vitro function of pancreatic islets.

Students' Scientific Research Centre, Tehran University of Medical Science, Tehran 1411413137, Iran.
Transplantation Proceedings (impact factor: 1). 12/2009; 41(10):4313-5. DOI:10.1016/j.transproceed.2009.09.065 pp.4313-5
Source: PubMed

ABSTRACT Although islet isolation and transplantation techniques have improved extensively in recent years, the loss of healthy functional islets is one of the major obstacles in this enterprise. A biostimulatory effect of low-level laser irradiation has been proven on proliferation of some kinds of cells. The aim of this study was to evaluate the effect of low-level laser irradiation on the function of isolated rat pancreatic islets after 24 hours of preculture.
Pancreatic islets isolated from male rats (250 to 300 g) were cultured for 24 hours in RPMI 1640 media. Groups of islets then received different energy densities (1, 3, 5 joules/cm(2) or silent) at 2 wavelengths (810 nm and 630 nm) using laser devices. Insulin concentrations in buffer media were measured as indices of islet function.
Irradiation of incubated islets with 830 nm low-level laser significantly increased insulin secretion after a glucose challenge test (P < .05). There was a significant increase in insulin secretion after irradiation with joules/cm(2) 630 nm energy density (P < .001).
These findings suggest that low-level laser irradiations improved islet cell function before transplantation.

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Keywords

2 wavelengths
 
830 nm low-level laser
 
biostimulatory effect
 
different energy densities
 
glucose challenge test
 
healthy functional islets
 
incubated islets
 
Insulin concentrations
 
islet cell function
 
islet function
 
islet isolation
 
islets
 
low-level laser irradiation
 
low-level laser irradiations
 
major obstacles
 
male rats
 
Pancreatic islets
 
rat pancreatic islets
 
recent years
 
transplantation techniques