Multiparameter Screening on SlipChip Used for Nanoliter Protein Crystallization Combining Free Interface Diffusion and Microbatch Methods

Institute for Biophysical Dynamics, University of Chicago, Chicago, Illinois, United States
Journal of the American Chemical Society (Impact Factor: 12.11). 12/2009; 132(1):112-9. DOI: 10.1021/ja908558m
Source: PubMed


This paper describes two SlipChip-based approaches to protein crystallization: a SlipChip-based free interface diffusion (FID) method and a SlipChip-based composite method that simultaneously performs microbatch and FID crystallization methods in a single device. The FID SlipChip was designed to screen multiple reagents, each at multiple diffusion equilibration times, and was validated by screening conditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofolate reductase/thymidylate synthase from Babesia bovis, against 48 different reagents at five different equilibration times each, consuming 12 microL of each protein for a total of 480 experiments using three SlipChips. The composite SlipChip was designed to screen multiple reagents, each at multiple mixing ratios and multiple equilibration times, and was validated by screening conditions for crystallization of two proteins, enoyl-CoA hydratase from Mycobacterium tuberculosis and dihydrofolate reductase/thymidylate synthase from Babesia bovis. To prevent cross-contamination while keeping the solution in the neck channels for FID stable, the plates of the SlipChip were etched with a pattern of nanowells. This nanopattern was used to increase the contact angle of aqueous solutions on the surface of the silanized glass. The composite SlipChip increased the number of successful crystallization conditions and identified more conditions for crystallization than separate FID and microbatch screenings. Crystallization experiments were scaled up in well plates using conditions identified during the SlipChip screenings, and X-ray diffraction data were obtained to yield the protein structure of dihydrofolate reductase/thymidylate synthase at 1.95 A resolution. This free-interface diffusion approach provides a convenient and high-throughput method of setting up gradients in microfluidic devices and may find additional applications in cell-based assays.

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Available from: Wenbin Du, Aug 11, 2015
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    • "In recent years, multiphase microfluidics technique has been widely applied in diverse fields such as analytical chemistry, chemical synthesis, combinatorial analysis, protein crystallization and drug discovery (Dendukuri and Doyle, 2009; Gunther and Jensen, 2006; Huebner et al., 2008; Li et al., 2010; Marre and Jensen, 2010; Song et al., 2006; Teh et al., 2008; Theberge et al., 2010; Whitesides, 2006). This technique is typically characterized by the transport of two or more immiscible fluids through microchannels with the cross-sectional dimension in the order of 100 μm. "
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    Journal of the American Chemical Society 12/2009; 132(1):106-11. DOI:10.1021/ja908555n · 12.11 Impact Factor
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