An adult tissue-specific stem cell in its niche: A gene profiling analysis of in vivo quiescent and activated muscle satellite cells

Molecular Genetics of Development Unit, Department of Developmental Biology, URA CNRS 2578, Institut Pasteur, Paris, France.
Stem Cell Research (Impact Factor: 3.91). 10/2009; 4(2):77-91. DOI: 10.1016/j.scr.2009.10.003
Source: PubMed

ABSTRACT The satellite cell of skeletal muscle provides a paradigm for quiescent and activated tissue stem cell states. We have carried out transcriptome analyses on satellite cells purified by flow cytometry from Pax3(GFP/+) mice. We compared samples from adult skeletal muscles where satellite cells are mainly quiescent, with samples from growing muscles or regenerating (mdx) muscles, where they are activated. Analysis of regulation that is shared by both activated states avoids other effects due to immature or pathological conditions. This in vivo profile differs from that of previously analyzed satellite cells activated after cell culture. It reveals how the satellite cell protects itself from damage and maintains quiescence, while being primed for activation on receipt of the appropriate signal. This is illustrated by manipulation of the corepressor Dach1, and by the demonstration that quiescent satellite cells are better protected from oxidative stress than those from mdx or 1-week-old muscles. The quiescent versus in vivo activated comparison also gives new insights into how the satellite cell controls its niche on the muscle fiber through cell adhesion and matrix remodeling. The latter also potentiates growth factor activity through proteoglycan modification. Dismantling the extracellular matrix is important for satellite cell activation when the expression of proteinases is up-regulated, whereas transcripts for their inhibitors are high in quiescent cells. In keeping with this, we demonstrate that metalloproteinase function is required for efficient regeneration in vivo.

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    • "*p < 0.05 **p < 0.01 ***p < 0.001. activated in EOMs (data not shown) and consistent with observations in TA and other skeletal muscles (Pallafacchina et al., 2010) but in contrast with previous reports in EOMs (McLoon and Wirtschafter, 2002a,b, 2003; McLoon et al., 2004). This likely reflects the different animal species and ages considered (rabbits, mice, rats) as well as the different techniques used to reveal satellite cell activation and proliferation (i.e., single myofiber reconstruction vs. histological analysis of transversal sections) (McLoon and Wirtschafter, 2002a,b; McLoon et al., 2004). "
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    ABSTRACT: Specific muscles are spared in many degenerative myopathies. Most notably, the extraocular muscles (EOMs) do not show clinical signs of late stage myopathies including the accumulation of fibrosis and fat. It has been proposed that an altered stem cell niche underlies the resistance of EOMs in these pathologies, however, to date, no reports have provided a detailed characterization of the EOM stem cell niche. PW1/Peg3 is expressed in progenitor cells in all adult tissues including satellite cells and a subset of interstitial non-satellite cell progenitors in muscle. These PW1-positive interstitial cells (PICs) include a fibroadipogenic progenitor population (FAP) that give rise to fat and fibrosis in late stage myopathies. PICs/FAPs are mobilized following injury and FAPs exert a promyogenic role upon myoblasts in vitro but require the presence of a minimal population of satellite cells in vivo. We and others recently described that FAPs express promyogenic factors while satellite cells express antimyogenic factors suggesting that PICs/FAPs act as support niche cells in skeletal muscle through paracrine interactions. We analyzed the EOM stem cell niche in young adult and aged wild-type mice and found that the balance between PICs and satellite cells within the EOM stem cell niche is maintained throughout life. Moreover, in the adult mdx mouse model for Duchenne muscular dystrophy (DMD), the EOM stem cell niche is unperturbed compared to normal mice, in contrast to Tibialis Anterior (TA) muscle, which displays signs of ongoing degeneration/regeneration. Regenerating mdx TA shows increased levels of both PICs and satellite cells, comparable to normal unaffected EOMs. We propose that the increase in PICs that we observe in normal EOMs contributes to preserving the integrity of the myofibers and satellite cells. Our data suggest that molecular cues regulating muscle regeneration are intrinsic properties of EOMs.
    Frontiers in Aging Neuroscience 12/2014; 6:328. DOI:10.3389/fnagi.2014.00328 · 2.84 Impact Factor
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    • "Regeneration is a multifactorial process that involves multiple cell types and a functional niche (Boldrin, et al., 2012; Saclier, et al., 2013; Yin et al., 2013). The niche might not have protective properties for highly doses of strongly penetrating IR used here (Reisz et al., 2014), as it appears to be for endogenous oxidative stress (Pallafacchina et al., 2010). Interestingly, thrombopoietin a factor of the hematopoietic niche, has been shown to stimulate DNAPKcsdependent repair in HSPCs (de Laval et al., 2013). "
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    ABSTRACT: The loss of genome integrity in adult stem cells results in accelerated tissue aging and possibly cancerogenesis. Adult stem cells in different tissues appear to react robustly to DNA damage. We report that adult skeletal stem (satellite) cells do not primarily respond to radiation-induced DNA double-strand breaks (DSBs) via differentiation and exhibit less apoptosis compared to other myogenic cells. Satellite cells repair these DNA lesions more efficiently than their committed progeny. Importantly, non-proliferating satellite cells and post-mitotic nuclei in the fibre exhibit dramatically distinct repair efficiencies. Altogether, reduction of the repair capacity appears to be more a function of differentiation than of the proliferation status of the muscle cell. Notably, satellite cells retain high efficiency of DSB repair also when isolated from the natural niche. Finally, we show that repair of DSB substrates is not only very efficient but, surprisingly, also very accurate in satellite cells and that accurate repair depends on the key non-homologous end-joining factor DNA-PKcs.
    Stem Cell Research 08/2014; 13(3). DOI:10.1016/j.scr.2014.08.005 · 3.91 Impact Factor
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    • "When activated , they start proliferating and can generate in few days a large number of myofibers: it has been demonstrated that few as seven satellite cells transplanted into irradiated muscle of dystrophic immune-deficient mice led to an increase of 100 new muscle fibers with thousands of myonuclei (Collins et al., 2005). Activated satellite cells cultured on plastic collagen-coated dishes are named " satellite cell-derived myoblasts " or myogenic precursor cells distinct from a functional and molecular point of view from freshly isolated satellite cells, possibly due to the absence of their niche (Dhawan and Rando, 2005; Pallafacchina et al., 2010). Stem cells can self-renew in asymmetric and symmetric "
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    ABSTRACT: Sphingosine 1-phosphate (S1P) is a bioactive lipid involved in the regulation of biological processes such as proliferation, differentiation, motility, and survival. Here we review the role of S1P in the biology and homeostasis of skeletal muscle. S1P derives from the catabolism of sphingomyelin and is produced by sphingosine phosphorylation catalyzed by sphingosine kinase (SK). S1P can act either intracellularly or extracellularly through specific ligation to its five G protein-coupled receptors (GPCR) named S1P receptors (S1PR). Many experimental findings obtained in the last 20 years demonstrate that S1P and its metabolism play a multifaceted role in the regulation of skeletal muscle regeneration. Indeed, this lipid is known to activate muscle-resident satellite cells, regulating their proliferation and differentiation, as well as mesenchymal progenitors such as mesoangioblasts that originate outside skeletal muscle, both involved in tissue repair following an injury or disease. The molecular mechanism of action of S1P in skeletal muscle cell precursors is highly complex, especially because S1P axis is under the control of a number of growth factors and cytokines, canonical regulators of skeletal muscle biology. Moreover, this lipid is crucially involved in the regulation of skeletal muscle contractile properties, responsiveness to insulin, fatigue resistance and tropism. Overall, on the basis of these findings S1P signaling appears to be an appealing pharmacological target for improving skeletal muscle repair. Nevertheless, further understanding is required on the regulation of S1P downstream signaling pathways and the expression of S1PR. This article will resume our current knowledge on S1P signaling in skeletal muscle, hopefully stimulating further investigation in the field, aimed at individuating novel molecular targets for ameliorating skeletal muscle regeneration and reducing fibrosis of the tissue after a trauma or due to skeletal muscle diseases.
    Frontiers in Physiology 11/2013; 4:338. DOI:10.3389/fphys.2013.00338 · 3.50 Impact Factor
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