Overexpression of the NAC transcription factor family gene ANAC036 results in a dwarf phenotype in Arabidopsis thaliana.
ABSTRACT NAC proteins comprise one of the largest families of transcription factors in the plant genome. They are known to be involved in various aspects of plant development, but the functions of most of them have not yet been determined. ANAC036, a member of the Arabidopsis NAC transcription factor family, contains unique sequences that are conserved among various NAC proteins found in other plant species. Expression analysis of the ANAC036 gene indicated that this gene was strongly expressed in leaves. Transgenic plants overexpressing the ANAC036 gene showed a semidwarf phenotype. The lengths of leaf blades, petioles and stems of these plants were smaller than those in wild-type plants. Microscopy revealed that cell sizes in leaves and stems of these plants were smaller than those in wild-type plants. These findings suggested that ANAC036 and its orthologues are involved in the growth of leaf cells.
- SourceAvailable from: Fernando C Gómez-Merino[Show abstract] [Hide abstract]
ABSTRACT: Osmotic stress caused by cold, salinity and drought are among the major stresses, which adversely affect plant growth and productivity worldwide. Plant acclimation to osmotic stress depends on regulation of biochemical and molecular networks involved in stress perception, signal transduction and expression of specific genes related to such environmental restrictions. The key components controlling and modulating stress acclimation pathways are transcription factors, small proteins encoded by single genes that regulate expression of many other genes, leading to the modulation of complex acclimation mechanisms, Transcription factors represent a major target for understanding the mechanisms used by plants to develop tolerance against these kinds of environmental constraints. In higher plants with complete genome sequences, the number of transcription factor families is large and varies between 79 y 81, depending on the species. The objective of this review is to analyze the role of transcription factors in the molecular response mechanisms to osmotic stress, with emphasis on the NAC (NAM, ATAF1-2 and CUC) family and its role in the regulation of plant responses to osmotic stress.Revista fitotecnia mexicana publ. por la Sociedad Mexicana de Fitogenética 05/2013; 36(2):105 - 115. · 0.26 Impact Factor
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ABSTRACT: NAM, ATAF, and CUC (NAC) genes are plant-specific transcription factors (TFs) that play key roles in plant growth, development, and stress tolerance. To date, none of the ramie NAC (BnNAC) genes had been identified, even though ramie is one of the most important natural fiber crops. In order to mine the BnNAC TFs and identify their potential function, the search for BnNAC genes against two pools of unigenes de novo assembled from the RNA-seq in our two previous studies was performed, and a total of 32 full-length BnNAC genes were identified in this study. Forty-seven function-known NAC proteins published in other species, in concert with these 32 BnNAC proteins were subjected to phylogenetic analysis, and the result showed that all the 79 NAC proteins can be divided into eight groups (NAC-I-VIII). Among the 32 BnNAC genes, 24, 2, and 1 gene showed higher expression in stem xylem, leaf, and flower, respectively. Furthermore, the expression of 14, 11 and 4 BnNAC genes was regulated by drought, cadmium stress, and infection by root lesion nematode, respectively. Interestingly, there were five BnNAC TFs which showed high homology with the NAC TFs of other species involved in regulating the secondary wall synthesis, and their expressions were not regulated by drought and cadmium stress. These results suggested that the BnNAC family might have a functional diversity. The identification of these 32 full-length BnNAC genes and the characterization of their expression pattern provide a basis for future clarification of their functions in ramie growth and development.MGG Molecular & General Genetics 04/2014; · 2.58 Impact Factor
Dataset: RFM Factores de Transcripción 2013