Microfluidic chips for the molecular analysis of human cancer
ABSTRACT Although the large-scale analysis of the human genome has provided a wealth of information for the genetic analysis of cancer and other diseases, most of these advances are unavailable in the clinic due to their expense and complexity. The development of miniaturized devices capable of automated real time analysis of genetic profiles is likely to enable routine genetic analysis of diseases such as cancer, whether for diagnosis or for monitoring treatment throughout the course of the disease. Microfluidic chips allow detection of mutations and abnormal gene expression patterns. Here, we describe the application of microfluidic chips for the molecular monitoring of gene expression profiles associated with human cancer. On-chip RT-PCR products are detectable after as few as 15 cycles of PCR, and from individual cells. On-chip detection is as sensitive as or exceeds the sensitivity obtained using conventional technologies.
Conference Proceeding: Modeling and controller design of a nonlinear time-varying thermal device in a microfluidic platform[show abstract] [hide abstract]
ABSTRACT: A switching controller is designed and implemented in a custom-made temperature control system to perform sensitive bio-chemical reactions for medical diagnostics within a microfluidic platform. Nonlinear and time-varying characteristics of the plant are considered, and the system is approximated by multi-linear models. Both simulation and experimental results demonstrate that the switching controller exhibits superior performance compared with other controllers considered. The efficacy of this design is exhibited by the application to a clinically important diagnostic test - polymerase chain reaction (PCR)American Control Conference, 2006; 07/2006