Article

Attraction of colorado potato beetle to herbivore-damaged plants during herbivory and after its termination.

Agriculture and Agri-food Canada
Journal of Chemical Ecology (Impact Factor: 2.24). 04/1997; 23:1003-1023. DOI: 10.1023/B:JOEC.0000006385.70652.5e
Source: OAI

ABSTRACT Large, undamaged potato plants (> 60 cm, 5-6 weeks old) attract the Colorado potato beetle (Leptinotarsa decemlineata), but small potato plants (15-25 cm high, 2-3 weeks old) do not. However, small plants become attractive to CPB when they are damaged. Mechanical damage inflicted with scissors results in short-term (lasting less than 15 min) attraction, while more severe damage with carborundum powder results in a longer lasting attraction (at least 1 hr), CPB adults are also attracted to small plants infested with CPB and Spodoptera exigua larvae. After the larvae had been removed for 50 min following a short duration (30 min) of feeding, CPB adults were no longer attracted to the plants. However, when CPB larvae had been removed after they had fed for 60-90 min, the plants were somewhat attractive to the beetles, although significantly less than they had been when the larvae were feeding. Attraction increased with time after feeding ceased. Furthermore, beetles were strongly attracted to plants 50 min after larvae were removed when the plants had been fed upon by larvae for 18-24 hr. Thus it appears that there are two stages of attraction, first, to volatiles released directly from the wound site, and second, to volatiles that are induced in response to herbivory. Chemical analyses of the headspace of infested potato plants show that infestation results in the emission of a mixture of chemicals that is qualitatively quite similar to that emitted by undamaged plants. The major components of the mixture are that emitted by undamaged plants. The major components of the mixture are terpenoids and fatty acid derivatives such as aldehydes and alcohols. The emission rate of some of these chemicals declines after removal of the beetles, while the emission rate of other chemical:, increases with the duration of beetle feeding and remains at a high level even after removal of the beetles. Thus, the composition of the mixture changes temporally during and after herbivore feeding, which may explain the recorded behavior of the beetles.

1 Bookmark
 · 
256 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Marking biological control agents facilitates studies of dispersal and predation. This study examines the effect of a biological solvent, dimethyl sulfoxide (DMSO), on retention of immunoglobulin G (IgG) protein solutions applied to Diorhabda carinulata (Desbrochers) (Coleoptera: Chrysomelidae), an important biological control agent of saltcedar, either internally by feeding them protein-labeled foliage or externally by immersing them in a protein solution. In addition, we determined whether internally or externally marked DMSO-IgG labels could be transferred via feeding from marked D. carinulata to its predator, Perillus bioculatus (Fabricius) (Heteroptera: Pentatomidae). The presence of rabbit and chicken IgG proteins was detected by IgG-specific enzyme-linked immunosorbent assays (ELISA). DMSO-IgG treatments showed greater label retention than IgG treatments alone, and this effect was stronger for rabbit IgG than for chicken IgG. Fourteen days after marking, beetles immersed in rabbit IgG showed 100% internal retention of label, whereas beetles immersed in chicken IgG showed 65% internal retention. Immersion led to greater initial (time 0) label values, and longer label retention, than feeding beetles labeled foliage. The DMSO-IgG label was readily transferred to P. bioculatus after feeding on a single marked prey insect. This investigation shows that addition of DMSO enhances retention of IgG labels, and demonstrates that protein marking technology has potential for use in dispersal and predator–prey studies with D. carinulata. Moreover, our observation of P. bioculatus feeding on D. carinulata is, to our knowledge, a new predator–prey association for the stink bug.
    Entomologia Experimentalis et Applicata 09/2013; 148(3). · 1.71 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Brontispa longissima (Gestro) (Coleoptera: Hispidae) is a new invasive pest in China that has caused severe economic damage to palm trees (Arecaceae, Palmae). The response of this beetle to coconut palm (Cocos nucifera) leaf volatiles is investigated in laboratory bioassays. Both sexes are attracted to a mixture of β-myrcene, (−)-limonene and E-2-hexen-1-ol (1 : 6 : 1), which are key components of coconut palm leaf volatiles. A blend of β-myrcene and (−)-limonene (0.7 : 1–1 : 0.7) in low amounts (100 ng) elicits aggregation and oviposition in females. Chemical analyses of food-deprived, gravid female B. longissima show high concentrations of β-myrcene and (−)-limonene in their accessory glands, suggesting that female beetles sequester both compounds and release them during oviposition.
    Physiological Entomology 12/2011; 36(4). · 1.43 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In the field, plants are challenged by more than one biotic stressor at the same time. In the present study, the molecular interactions between potato (Solanum tuberosum L.), Colorado potato beetle (Leptinotarsa decemlineata Say; CPB), and Potato virus YNTN (PVYNTN) were investigated through analyses of gene expression in the potato leaves and the gut of the CPB larvae, and of the release of potato volatile compounds. CPB larval growth was enhanced when reared on secondary PVYNTN-infected plants, which was associated with decreased accumulation of transcripts associated with the antinutritional properties of potato. In PVYNTN-infected plants, ethylene signalling pathway induction and induction of auxin response transcription factors was attenuated, while no differences were observed in JA signalling pathway. Similarly to rearing on virus-infected plants, CPB larvae gained more weight when reared on plants silenced in JA receptor gene (coi1). Although herbivore induced defence mechanism is regulated predominantly by JA, response in coi1-silenced plants only partially corresponded to the one observed in PVYNTN-infected plants, confirming the role of other plant hormones in modulating this response. The release of β–barbatene and benzyl alcohol was different in healthy and PVYNTN-infected plants before CPB larvae infestation, implicating the importance of PVYNTN infection in plant communication with its environment. This was reflected in gene expression profiles of neighbouring plants showing different degree of defence response. The present study thus contributes to our understanding of plant responses in agro-ecosystems.This article is protected by copyright. All rights reserved.
    Molecular Ecology 09/2014; · 5.84 Impact Factor

Full-text (2 Sources)

Download
378 Downloads
Available from
May 22, 2014