Assessment of phenolic content, free-radical-scavenging capacity genotoxic and anti-genotoxic effect of aqueous extract prepared from Moricandia arvensis leaves.
ABSTRACT The present study was undertaken to provide a set of data on the safety of an aqueous extract (AQE) from Moricandia arvensis. For this reason, Escherichia coli tested strains PQ35 and PQ37 were used to detect induction of DNA lesions by AQE. The SOS Chromotest showed that AQE induced a marginally genotoxic effect, as expressed by the induction factor (IF) value only with E. coli PQ37 tested strain (IF=1.77 at a dose of 250 microg/assay). The measurement of the anti-genotoxic activity of the AQE was also studied by inhibition of beta-galactosidase induction. A significant anti-genotoxic effect was observed with different tested doses of AQE, which suggests that M. arvensis extract has the potential to protect DNA from the action of nitrofurantoïn (NF) and free radicals generated by hydrogen peroxide (H2O2). In addition to anti-genotoxic activity, AQE showed a free-radical-scavenging capacity towards ABTS+* and DPPH*. Total phenolic content was also evaluated following Folin-Ciocalteu method and results indicated high correlation between total phenol content and anti-genotoxic and antioxidant activities for AQE, but the highest correlation was showed with its capacity to stabilize ABTS+* (R2=0.9944).
- Food and Chemical Toxicology - FOOD CHEM TOXICOL. 01/2008; 7(4):21-21.
Article: Moricandia[Show abstract] [Hide abstract]
ABSTRACT: Moricandia sp. is a coenospecies of the Brassicaceae family originating in the semi-arid Mediterranean regions. Utilized as an ornamental species and consumed in some regions of Spain, Moricandia spp. are not commonly grown for cultivation. M arvensis has been studied for antioxidant and antigenotoxic compounds that may be useful in the fields of nutraceutical research. Interspecific crosses of Moricandia with commonly cultivated Brassica species have been used for breeding and research purposes. This has allowed the development of cytoplasmic male sterility and restorer lines for hybrid B. juncea production. Moricandia has C3 and C3–C4 intermediate photosynthetic phenotypes, which have been used in the study of C4 evolution and the attempted introduction of intermediate C3–C4 physiology into cultivated Brassica sp. to attempt to improve drought tolerance.
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ABSTRACT: The aims of this study were to investigate the antioxidant (radical scavenging, reducing) and antiproliferative potential of a methanolic extract (ME) of Aloe vera leaves. DPPH radical scavenging activity was ME—19% (1250 μg/mL); positive controls, ascorbic acid and BHA—24 and 31%, respectively (12.5 μg/mL); 63 and 62%, respectively (50 μg/mL). ABTS+ radical-scavenging activity of 0.046 mM Trolox (TEAC) was equal to that of 1 mg/mL of ME. Protection of supercoiled pET20b(+) DNA from hydroxyl radicals was as follows: 2000 μg/mL quercetin > 5000 μg/mL ME > 2000 μg/mL ME. Thin layer chromatography (TLC) was used to qualitatively determine whether the components responsible for antioxidant activity of ME included phenols and anthraquinones. The pre-normalized total antioxidant activity of ME ranged from 23% (125 μg/mL) to 47% (500 μg/mL) for ME vs. 90% (312.5 μg/mL) for ascorbic acid. The flavonoid and phenolic contents of ME were 14.1 ± 1.6 CE/g and 38.9 ± 7.6 GAE/g, respectively. The growth inhibition by ME (100 μg/mL) was 32.6, 20.8, 10.6, and -5% in LnCaP, A549, MG63, and HCT15, respectively. To the best of our knowledge, this is the first reported investigation of antiproliferative activity of an Aloe vera extract in these human cancer cell lines, whose complex interactions with the ME components translate into the observed differences in growth inhibition and thus warrant further study.The Journal of Pharmacy 08/2011; 4(8):2791-2796.