Assessment of phenolic content, free-radical-scavenging capacity genotoxic and anti-genotoxic effect of aqueous extract prepared from Moricandia arvensis leaves.
ABSTRACT The present study was undertaken to provide a set of data on the safety of an aqueous extract (AQE) from Moricandia arvensis. For this reason, Escherichia coli tested strains PQ35 and PQ37 were used to detect induction of DNA lesions by AQE. The SOS Chromotest showed that AQE induced a marginally genotoxic effect, as expressed by the induction factor (IF) value only with E. coli PQ37 tested strain (IF=1.77 at a dose of 250 microg/assay). The measurement of the anti-genotoxic activity of the AQE was also studied by inhibition of beta-galactosidase induction. A significant anti-genotoxic effect was observed with different tested doses of AQE, which suggests that M. arvensis extract has the potential to protect DNA from the action of nitrofurantoïn (NF) and free radicals generated by hydrogen peroxide (H2O2). In addition to anti-genotoxic activity, AQE showed a free-radical-scavenging capacity towards ABTS+* and DPPH*. Total phenolic content was also evaluated following Folin-Ciocalteu method and results indicated high correlation between total phenol content and anti-genotoxic and antioxidant activities for AQE, but the highest correlation was showed with its capacity to stabilize ABTS+* (R2=0.9944).
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ABSTRACT: Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts. The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF) enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the "SOS chromotest test". Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase. None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay. The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules.BMC Complementary and Alternative Medicine 09/2012; 12:153. · 2.08 Impact Factor
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ABSTRACT: Exposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column derivatisation, and their potential ability to enhance DNA repair in human lymphocytes after hydrogen peroxide (H(2)O(2)) treatment (250 μM, 5 min). We also studied DNA repair in human kidney HEK 293 cells after exposure to 20 nm silver nanoparticles (AgNPs) (100 μg/ml, 30 min) in the presence and absence of the plant extract. We have found that mangiferin along with unidentified polar compounds are the most pronounced antioxidants in the studied extracts. Extract from haulm exhibited slightly stronger antioxidant properties compared to flower extracts. However, all four extracts showed significant ability to enhance DNA repair in both cell types after H(2)O(2) and AgNP treatments.Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 06/2012; 50(9):3352-9. · 2.99 Impact Factor
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ABSTRACT: The aims of this study were to investigate the antioxidant (radical scavenging, reducing) and antiproliferative potential of a methanolic extract (ME) of Aloe vera leaves. DPPH radical scavenging activity was ME—19% (1250 μg/mL); positive controls, ascorbic acid and BHA—24 and 31%, respectively (12.5 μg/mL); 63 and 62%, respectively (50 μg/mL). ABTS+ radical-scavenging activity of 0.046 mM Trolox (TEAC) was equal to that of 1 mg/mL of ME. Protection of supercoiled pET20b(+) DNA from hydroxyl radicals was as follows: 2000 μg/mL quercetin > 5000 μg/mL ME > 2000 μg/mL ME. Thin layer chromatography (TLC) was used to qualitatively determine whether the components responsible for antioxidant activity of ME included phenols and anthraquinones. The pre-normalized total antioxidant activity of ME ranged from 23% (125 μg/mL) to 47% (500 μg/mL) for ME vs. 90% (312.5 μg/mL) for ascorbic acid. The flavonoid and phenolic contents of ME were 14.1 ± 1.6 CE/g and 38.9 ± 7.6 GAE/g, respectively. The growth inhibition by ME (100 μg/mL) was 32.6, 20.8, 10.6, and -5% in LnCaP, A549, MG63, and HCT15, respectively. To the best of our knowledge, this is the first reported investigation of antiproliferative activity of an Aloe vera extract in these human cancer cell lines, whose complex interactions with the ME components translate into the observed differences in growth inhibition and thus warrant further study.The Journal of Pharmacy 08/2011; 4(8):2791-2796.