Article

Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed.

Vectron Biosolutions AS, Pirsenteret, 7462 Trondheim, Norway.
Applied Microbiology and Biotechnology (impact factor: 3.42). 11/2009; 85(5):1301-13. DOI:10.1007/s00253-009-2349-1 pp.1301-13
Source: PubMed

ABSTRACT Methods for transfer of exogenous DNA into cells are essential for genetics and molecular biology, and the lack of effective methods hampers research on many different species of bacteria which have shown to be particularly recalcitrant to transformation. This review presents the progress on the development of methods for artificial transformation of bacteria with emphasis on different methodologies and the range of bacteria that can be transformed. The methods' strengths and weaknesses are described.

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Keywords

bacteria
 
different methodologies
 
different species
 
effective methods hampers research
 
methods' strengths
 
molecular biology
 
recalcitrant
 
weaknesses
 

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