Interlaboratory comparability of serum cotinine measurements at smoker and nonsmoker concentration levels: A round-robin study

Division of Laboratory Science , National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA.
Nicotine & Tobacco Research (Impact Factor: 3.3). 12/2009; 11(12):1458-66. DOI: 10.1093/ntr/ntp161
Source: PubMed


Cotinine, the primary proximate metabolite of nicotine, is commonly measured as an index of exposure to tobacco in both active users of tobacco and nonsmokers with possible exposure to secondhand smoke (SHS). A number of laboratories have implemented analyses for measuring serum cotinine in recent years, but there have been few interlaboratory comparisons of the results. Among nonsmokers exposed to SHS, the concentration of cotinine in blood can be quite low, and extensive variability in these measurements has been reported in the past.
In this study, a group of seven laboratories, all experienced in serum cotinine analysis, measured eight coded serum pools with concentrations ranging from background levels of about 0.05 ng/ml to relatively high concentrations in the active smokers range. All laboratories used either gas-liquid chromatography with nitrogen-phosphorus detection or liquid chromatography with mass spectrometric detection.
All seven laboratories reliably measured the cotinine concentrations in samples that were within the range of their methods. In each case, the results for the pools were correctly ranked in order, and no significant interlaboratory bias was observed at the 5% level of significance for results from any of the pools.
We conclude that present methods of chromatographic analysis of serum cotinine, as used by these experienced laboratories, are capable of providing accurate and precise results in both the smoker and the nonsmoker concentration range.

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    • "To account for previously observed associations between polychlorinated biphenyls (PCBs) and THs (Chevrier et al., 2007), we measured PCB-153 concentrations in maternal serum samples collected at 16 weeks gestation (Sjodin et al., 2014). Tobacco smoke exposure at 16 weeks was assessed using serum cotinine, a sensitive and specific biomarker of secondhand and active tobacco smoke exposure, using previously described methods (Bernert et al., 2009). Urinary iodine was measured in maternal urine collected at 26 (n ¼228) or 16 weeks (n ¼8) with an Agilent 7500x Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) (Caldwell et al., 2003). "
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    Environmental Research 03/2015; 138:453-460. DOI:10.1016/j.envres.2015.03.003 · 4.37 Impact Factor
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    • "Women provided nonfasting urine specimens upon enrollment. Serum cotinine was measured with high-performance liquid chromatography/tandem MS utilizing an isotope dilution method and external standard calibration plots (Bernert et al., 2009). Serum cotinine was categorized as nonsmoking (0–9.99 ng/mL) and passive/active smoking (10.00–595.31 "
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    • "× TC) + TG + 62.3 mg/dL, where TC denotes total cholesterol and TG denotes triglycerides, and were reported in milli grams per deciliter (Philips et al. 1989). Serum cotinine was quantified using highperformance liquid chromatography/tandem MS using an isotope dilution method and external standard calibration plots (Bernert et al. 2009). Serum cotinine was further categorized as noted above to help identify passive and active exposure using established cut-points (Wall et al. 1988). "
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