Article

Follicular fluid high density lipoprotein-associated micronutrient levels are associated with embryo fragmentation during IVF

Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, 26 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA.
Journal of Assisted Reproduction and Genetics (Impact Factor: 1.77). 11/2009; 26(11-12):557-60. DOI: 10.1007/s10815-009-9367-x
Source: PubMed

ABSTRACT To investigate whether follicular fluid lipid-soluble micronutrients are associated with embryo morphology parameters during IVF.
Follicle fluid and oocytes were obtained prospectively from 81 women. Embryo morphology parameters were used as surrogate markers of oocyte health. HDL lipids and lipid-soluble micronutrients were analyzed by high-pressure liquid chromatography. Non-parametric bi-variate analysis and multivariable ordinal logistic regression models were employed to examine associations between biochemical and embryo morphology parameters.
Follicular fluid HDL cholesterol (r = -0.47, p < 0.01), alpha-tocopherol (r = -0.41, p < 0.01), delta-tocopherol (r = -0.38, p < 0.05) and beta-cryptoxanthine (r = -0.42, p < 0.01) are negatively correlated with embryo fragmentation. Ordinal logistic regression models indicate that a 0.1 mumol/L increase in beta-cryptoxanthine, adjusted for gamma-tocopherol, is associated with a 75% decrease in the cumulative odds of higher embryo fragmentation (p = 0.010).
Follicular fluid HDL micronutrients may play an important role in the development of the human oocyte as evident by embryo fragmentation during IVF.

Full-text

Available from: Michael S Bloom, Apr 28, 2015
0 Followers
 · 
82 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Context: A role of lipids in human fecundity is hypothesized as cholesterol is the main substrate for steroid synthesis and has also been shown to affect the hormonal milieu and steroidogenesis in both men and women. Objective: The objective of the study was to evaluate the association between male and female serum lipid concentrations and time to pregnancy (TTP). Design/Setting: A population-based prospective cohort study recruiting couples from 16 counties in Michigan and Texas (2005-2009) using sampling frameworks allowing for identification of couples planning pregnancy in the near future. Participants: Five hundred one couples desiring pregnancy and discontinuing contraception were followed up for 12 months or until an human chorionic gonadotropin pregnancy was detected. Main Outcome and Measures: Fecundability odds ratios (FORs) and 95% confidence intervals (CIs) were estimated after adjusting for age, body mass index, race, and education in relationship to female, male, and joint couple lipid concentrations. Results: Serum free cholesterol levels were higher on average among male and female partners of couples who did not became pregnant during the study follow-up (female, P = .04; male, P = .009), and levels in female partners were associated with significantly longer TTP in models based on both individual and couples concentrations (individual models: FOR 0.98, 95% CI 0.97, 0.99; couple models: FOR 0.98, 95% CI 0.97, 0.99). Male free cholesterol concentrations were associated with TTP only in the couple-based models (FOR 0.98, 95% CI 0.97, 0.99). Sensitivity analyses suggested that the observed associations are unlikely to be explained by potential unmeasured confounding such as diet. Conclusions: Our results suggest that serum free cholesterol concentrations in both men and women have an effect on TTP, highlighting the importance of cholesterol and lipid homeostasis for male and female fecundity.
    Journal of Clinical Endocrinology &amp Metabolism 05/2014; 99(8):jc20133936. DOI:10.1210/jc.2013-3936 · 6.31 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Metabolism and ATP levels within the oocyte and adjacent cumulus cells are associated with oocyte quality and optimal development of a healthy embryo. Lipid metabolism provides a potent source of energy and its importance during oocyte maturation is being increasingly recognised. The triglyceride and fatty acid composition of ovarian follicular fluid has been characterised for many species and is influenced by nutritional status (i.e. dietary fat, fasting, obesity and season) as well as lactation in cows. Lipid in oocytes is primarily triglyceride of specific fatty acids which differ by species, stored in distinct droplet organelles that re-localise during oocyte maturation. The presence of lipids, particularly saturated versus unsaturated fatty acids, in in vitro maturation (IVM) systems affects oocyte lipid content as well as developmental competence. Triglycerides are metabolised by lipases which have been localised to cumulus cells as well as oocytes. Fatty acids generated by lipolysis are further metabolised byβ-oxidation in mitochondria for the production of ATP.β-oxidation is induced in cumulus-oocyte complexes by the LH surge and pharmacological inhibition ofβ-oxidation impairs oocyte maturation and embryo development. Promotingβ-oxidation with L-carnitine improves embryo development many species. Thus fatty acid metabolism in the mammalian cumulus-oocyte complex is regulated by maternal physiological and in vitro environmental conditions; and is important for oocyte developmental competence.
    Reproduction 04/2014; DOI:10.1530/REP-13-0251
  • [Show abstract] [Hide abstract]
    ABSTRACT: During episodes of undernutrition and overnutrition the mammalian preimplantation embryo undergoes molecular and metabolic adaptations to cope with nutrient deficits or excesses. Maternal adaptations also take place to keep a nutritional microenvironment favorable for oocyte development and embryo formation. This maternal-embryo communication takes place via several nutritional mediators. Although adaptive responses to malnutrition by both the mother and the embryo may ensure blastocyst formation, the resultant quality of the embryo can be compromised, leading to early pregnancy failure. Still, studies have shown that, although early embryonic mortality can be induced during malnutrition, the preimplantation embryo possesses an enormous plasticity that allows it to implant and achieve a full-term pregnancy under nutritional stress, even in extreme cases of malnutrition. This developmental strategy, however, may come with a price, as shown by the adverse developmental programming induced by even subtle nutritional challenges exerted exclusively during folliculogenesis and the preimplantation period, resulting in offspring with a higher risk of developing deleterious phenotypes in adulthood. Overall, current evidence indicates that malnutrition during the periconceptional period can induce cellular and molecular alterations in preimplantation embryos with repercussions for fertility and postnatal health.
    Domestic Animal Endocrinology 12/2014; 51:27-45. DOI:10.1016/j.domaniend.2014.10.003 · 1.78 Impact Factor