Ultrafast Dynamics of Protein Proton Transfer on Short Hydrogen Bond Potential Energy Surfaces: S65T/H148D GFP.

School of Chemistry, University of East Anglia, Norwich NR4 7TJ, UK.
Journal of the American Chemical Society (Impact Factor: 12.11). 11/2009; 132(5):1452-3. DOI: 10.1021/ja907690g
Source: PubMed


Ultrafast proton transfer dynamics on a short H-bond in a protein were studied through the time-resolved fluorescence of the S65T/H148D green fluorescent protein (GFP) mutant. In response to the change in chromophore pK(a) upon excitation, the donor-proton-acceptor structure evolves on a sub-100 fs time scale, followed by picosecond time scale vibrational cooling and host structure reorganization.

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Available from: Ismael A Heisler, Aug 06, 2014
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