Vitrification of biopsied embryos at cleavage, morula and blastocyst stage

Pedieos IVF Center, Nicosia, Cyprus.
Reproductive biomedicine online (Impact Factor: 3.02). 10/2009; 19(4):526-31. DOI: 10.1016/j.rbmo.2009.05.009
Source: PubMed

ABSTRACT This study investigated the effect of vitrification on biopsied embryos at various developmental stages. After biopsy on day 3, embryos were vitrified at cleavage, morula and blastocyst stages using a commercially available kit. Nonbiopsied embryos were vitrified as controls. For day-3 cleavage embryo vitrification, embryos from abnormally fertilized oocytes were randomly allocated to the biopsy and control groups. For morula and blastocyst vitrification, the embryos used in the biopsy groups were obtained from aneuploidy or affected embryos diagnosed by preimplantation genetic diagnosis (PGD). After warming, survival, blastulation and development of embryos in different groups were compared. The survival rate after warming in the non-biopsied cleavage control group was significantly higher than in the biopsied cleavage group (92.0% versus 64.0%, P = 0.037). Most of the biopsied embryos were destroyed due to blastomeres escaping. At the morula stage, both biopsied and non-biopsied embryos had similar survival rates. However, a significantly higher survival rate (95.6%) was observed in the biopsied blastocyst group compared with the control group (81.3%, P = 0.035). Biopsied embryos vitrified at an advanced stage had as high survival rates as non-biopsied embryos. Vitrification at the blastocyst stage is a practical and efficient solution for embryo cryopreservation during PGD.

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    • "Potential sources of genetic material are: first (1 PB) and second polar bodies (2 PB), day 3 embryo blastomeres , and blastocyst trophectoderm cells (Brezina et al. 2013; Greco et al. 2013). All these techniques require several laboratory manipulation procedures before embryo transfer: oocyte zona pellucida laser drilling, embryo biopsy, cryopreservation and thawing, that might affect embryo survival and its implantation potential (Scott et al. 2013a; Zhang et al. 2009). "
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    ABSTRACT: Introduction: Preimplantation genetic diagnosis and/or screening (PGD/PGS) allow the assessment of the genetic health of an embryo before transferring it into the uterus. These techniques require the removal of cellular material (polar bodies, blastomere(s) or trophectoderm cells) in order to perform the proper genetic analysis. We report the implantation and live birth outcome of a vitrified-warmed blastocyst developed after triple biopsy and double vitrification procedures at oocyte, cleavage embryo and blastocyst stage.
    SpringerPlus 01/2015; 4(1):22. DOI:10.1186/s40064-015-0788-y
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    • "To summarize, vitrification clearly outruns slow freezing at blastocyst stage in terms of safety and efficacy. Not only that vitrification seems to work in " extreme " cases, such as vitrification after in vitro maturation of oocytes (Lee et al., 2007), biopsy (Zhang et al., 2009), zona-free (Hiraoka et al., 2007; Shu et al., 2010), or previously vitrified blastocysts (Peng et al., 2011), this method also perfectly works under aseptic conditions (Escribá et al., 2008; Vanderzwalmen et al., 2009; Kamath et al., 2011; Van Landuyt et al., 2011a; 2011b). Kuwayama et al (2005) compared an open (Cryotop) and a " single-straw " closed (CryoTip) system in terms of blastocyst survival after vitrification and they could not find any difference in survival (97% vs. 93%), pregnancy (59% vs. 51%), and deliveries (51% vs. 48%). "
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    ABSTRACT: Vitrification is a cryopreservation technique increasingly applied in clinical practice for cells and tissue. This review article focuses mainly on the efficiency of vitrification of human reproductive cells and tissue, by analysing the clinical results reported in the literature. The second aspect discussed is safety of vitrification procedure. Different procedures and different types of carriers can be used, and in some cases vitrification requires a direct contact between cell/tissue/carrier and liquid nitrogen; this causes concern regarding the safety of this cryopreservation technique. Although the risk of contamination during cryopreservation remains negligible, this article explains how to overcome the hypothetical risk of contamination when using different types of vitrification carriers, in order to satisfy all existing directives.
    06/2012; 3(1):22-40. DOI:10.1177/205891581200300104
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    • "The survival rate can, however, be improved by vitrification after incubating the cleavage-stage embryos for 6-8 h following biopsy (Zheng et al., 2005), or by cryopreservation in CJ3 medium (Stachecki et al., 2005). Recently higher survival rates of biopsied cleavage-stage embryos have been obtained by vitrification at the blastocyst stage (Magli et al., 2006; Zhang et al., 2009). "
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    ABSTRACT: Preimplantation genetic testing is an important application of IVF and has broad interest to reproductive medicine practitioners. Economic and medical considerations favor a universal and affordable access to IVF and PGD/PGS services for carrier couples of severe inherited genetic disorders. In addition to current methodologies, preimplantation testing is an expanding field poised to adopt cutting-edge genomic technologies for new advances in preventative medical care. Technologies for interrogating whole genomes via SNP microarrays and DNA sequencing are rapidly becoming more powerful and more cost-effective. It will soon be feasible to apply these genomic tools widely in reproductive medicine.
    In Vitro Fertilization - Innovative Clinical and Laboratory Aspects, 04/2012; , ISBN: 978-953-51-0503-9
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