Article

Detection of gene expression in embryonic tissues and stratified epidermis by in situ hybridization.

Developmental Skin Biology Section, NIH/NIAMS, Bethesda, MD, USA.
Methods in molecular biology (Clifton, N.J.) (Impact Factor: 1.29). 01/2010; 585:253-60. DOI: 10.1007/978-1-60761-380-0_17
Source: PubMed

ABSTRACT Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.

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