Article

Altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome.

Department of Behavioral Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
Neurogastroenterology and Motility (impact factor: 3.41). 11/2009; 22(5):512-9, e114-5. DOI:10.1111/j.1365-2982.2009.01427.x pp.512-9, e114-5
Source: PubMed

ABSTRACT The profile of intestinal organic acids in irritable bowel syndrome (IBS) and its correlation with gastrointestinal (GI) symptoms are not clear. We hypothesized in this study that altered GI microbiota contribute to IBS symptoms through increased levels of organic acids.
Subjects were 26 IBS patients and 26 age- and sex-matched controls. Fecal samples were collected for microbiota analysis using quantitative real-time polymerase chain reaction and culture methods, and the determination of organic acid levels using high-performance liquid chromatography. Abdominal gas was quantified by image analyses of abdominal X-ray films. Subjects completed a questionnaire for GI symptoms, quality of life (QOL) and negative emotion.
Irritable bowel syndrome patients showed significantly higher counts of Veillonella (P = 0.046) and Lactobacillus (P = 0.031) than controls. They also expressed significantly higher levels of acetic acid (P = 0.049), propionic acid (P = 0.025) and total organic acids (P = 0.014) than controls. The quantity of bowel gas was not significantly different between controls and IBS patients. Finally, IBS patients with high acetic acid or propionic acid levels presented with significantly worse GI symptoms, QOL and negative emotions than those with low acetic acid or propionic acid levels or controls.
These results support the hypothesis that both fecal microbiota and organic acids are altered in IBS patients. A combination of Veillonella and Lactobacillus is known to produce acetic and propionic acid. High levels of acetic and propionic acid may associate with abdominal symptoms, impaired QOL and negative emotions in IBS.

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Keywords

abdominal X-ray films
 
altered GI microbiota
 
culture methods
 
fecal microbiota
 
high-performance liquid chromatography
 
higher counts
 
IBS patients
 
image analyses
 
intestinal organic acids
 
irritable bowel syndrome
 
Irritable bowel syndrome patients
 
low acetic acid
 
microbiota analysis
 
organic acid levels
 
propionic acid levels
 
quantitative real-time polymerase chain reaction
 
results support
 
sex-matched controls
 
total organic acids
 
worse GI symptoms
 

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