Article

The first detection of Babesia EU1 and Babesia canis canis in Ixodes ricinus ticks (Acari, Ixodidae) collected in urban and rural areas in northern Poland.

Department of Tropical Parasitology, Interfaculty Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, Gdynia, Poland.
Polish journal of microbiology / Polskie Towarzystwo Mikrobiologów = The Polish Society of Microbiologists (Impact Factor: 0.77). 01/2009; 58(3):231-6.
Source: PubMed

ABSTRACT Ixodes ricinus, the most commonly observed tick species in Poland, is a known vector of such pathogenic microorganisms as TBE viruses, Borrelia burgdoferi sensu lato, Anaplasma phagocytophilum, Rickettsia helvetica, Babesia divergens and B. microti in our country. Our study aimed to find out whether this tick can also transmit other babesiae of medical and veterinary importance. DNA extracts of 1392 ticks (314 nymphs, 552 male and 526 female ticks) collected in urban and rural areas in the Pomerania province (northern Poland), were examined by nested PCR for the detection of Babesia spp., using outer primers: 5-22F and 1661R, and inner primers: 455-479F and 793-772R, targeting specific fragment of 18S rRNA gene. Overall, at least 1.6% ticks were found to be infected with babesial parasites. In the case of nymphs, the minimal prevalence was 0.6%, and it was approx. 3-times lower than in adults (1.9%). Percentages of infected males and females were comparable (2.0% vs. 1.7%). Sequences of 15/22 PCR-derived fragments of 18S rRNA gene demonstrated 100% similarities with the sequence of Babesia EUI (proposed name B. venatorum) (acc. no. AY046575) (n = 13) and with B. canis canis (acc. no. AY321119) (n = 2), deposited in the GenBank database. The partial 18S rDNA sequences of Babesia EUI and B. c. canis obtained by us from I. ricinus have been deposited in GenBank, accession nos. GQ325619 and GQ325620, respectively. The results obtained suggest the possible role of I. ricinus as a source of microorganisms, which have been identified as agents of human and canine babesiosis, respectively, in Europe. To our knowledge this is the first report on the occurrence of Babesia EUI and B. c. canis in I. ricinus in Poland.

0 Bookmarks
 · 
181 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: BACKGROUND: Lyme disease is commonly diagnosed in humans in Latvia, but up to date no studies have been performed to investigate its prevalence in dogs. The aim of this study was to evaluate if seroprevalence against B. burgdorferi sensu lato (B. burgdorferi s.l.) and co-expression of antibodies against B.burgdorferi s.l. and A. phagocytophilum is higher in dogs with clinical suspicion of tick-borne diseases compared to healthy dogs. FINDINGS: Venous blood was taken from healthy dogs (n=441) and dogs suspected to have borreliosis and/ or canine granulocytic anaplasmosis (n=29). The presence of antibodies was detected with SNAP 4Dx test (IDEXX, Westbrook, Maine, USA). The seroprevalence against B. burgdorferi s.l. in healthy dogs was 2.49% (11/441) and 36% (4/11) of seropositive dogs had antibodies against both of investigated bacteria. None of the dogs in sick dog group had detectable antibodies against B. burgdorferi s.l. CONCLUSIONS: We conclude that seroprevalence to B. burgdorferi s.l. in dogs in Latvia is low and that dogs with suspicion of tick-borne disease do not have higher B. burgdorferi s. l. seroprevalence than healthy dogs. Dogs that express antibodies against B. burgdorferi s.l. frequently co-express antibodies against A. phagocytophilum.
    Irish veterinary journal 05/2013; 66(1):9. · 0.44 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This is the first report of confirmed canine babesiosis in Latvia supporting the observed geographical expansion of this disease. Between 2009 and 2011 three dogs which have not traveled outside of Latvia were diagnosed with babesiosis. Hematological analysis and serological tests for granulocytic anaplasmosis, ehrlichiosis and borreliosis were negative (Idexx SNAP 4Dx test). Peripheral blood erythrocytes of the three dogs contained large Babesia that were identified as Babesia canis canis by PCR. Sequences of partial 18S rRNA gene were 98-100% similar to the sequences of B. canis canis isolated from dogs in other European countries. We conclude that these are the first autochthonous canine babesiosis cases reported from Latvia.
    Veterinary Parasitology 03/2013; · 2.38 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Risk assessment of tick-borne and zoonotic disease emergence necessitates sound knowledge of the particular microorganisms circulating within the communities of these major vectors. Assessment of pathogens carried by wild ticks must be performed without a priori, to allow for the detection of new or unexpected agents. We evaluated the potential of Next-Generation Sequencing techniques (NGS) to produce an inventory of parasites carried by questing ticks. Sequences corresponding to parasites from two distinct genera were recovered in Ixodes ricinus ticks collected in Eastern France: Babesia spp. and Theileria spp. Four Babesia species were identified, three of which were zoonotic: B. divergens, Babesia sp. EU1 and B. microti; and one which infects cattle, B. major. This is the first time that these last two species have been identified in France. This approach also identified new sequences corresponding to as-yet unknown organisms similar to tropical Theileria species. Our findings demonstrate the capability of NGS to produce an inventory of live tick-borne parasites, which could potentially be transmitted by the ticks, and uncovers unexpected parasites in Western Europe.
    PLoS Neglected Tropical Diseases 03/2014; 8(3):e2753. · 4.57 Impact Factor

Full-text

View
0 Downloads
Available from