Article

Acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus.

Department of Animal Health and Environment, Estonian University of Life Sciences, Tartu, Estonia.
Comparative immunology, microbiology and infectious diseases (Impact Factor: 2.11). 11/2009; 34(1):23-9. DOI: 10.1016/j.cimid.2009.10.005
Source: PubMed

ABSTRACT Bovine acute phase proteins (APPs), lipopolysaccharide binding protein (LBP), serum amyloid A (SAA), haptoglobin (Hp) and alpha(1)-acid glycoprotein (AGP) were evaluated as inflammatory markers during an outbreak of bovine respiratory disease (BRD) caused by bovine respiratory syncytial virus (BRSV). Calves (n = 10) presented mild to moderate signs of respiratory disease. Secondary bacterial infections, Pasteurella multocida and Mycoplasma dispar as major species, were detected in tracheobronchial lavage samples. Concentrations of SAA and LBP increased at week 1 had the highest values at week 3 and decreased at week 4 of outbreak. Some calves had high Hp concentrations at week 3, but AGP concentrations did not rise during respiratory disease. Higher SAA, LBP and Hp concentrations at a later stage of BRD (week 3) were associated with the low BRSV-specific IgG(1) production, suggesting that these calves had enhanced inflammatory response to the secondary bacterial infection. In conclusion, APPs (especially SAA and LBP) are sensitive markers of respiratory infection, and they may be useful to explore host response to the respiratory infections in clinical research.

0 Followers
 · 
112 Views
  • Source
    Revue de médecine vétérinaire 01/2010; 161(12):535-539. · 0.25 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study was undertaken to evaluate the effect of different pathological lung affections of buffaloes (Bubalus bubalis) on blood acute-phase proteins. A total number of 88 female buffaloes (4–7 years old) were subjected to study. Out of them, 63 animals had lung affections that were classified according to the gross and histopathological findings into pulmonary congestion and edema (N = 7), bronchiolitis and emphysema (N = 20), fibrinous bronchopneumonia (N = 22), and broncho-interstitial pneumonia (N = 14). The remained animals (N = 25) were kept as control. Plasma fibrinogen, serum haptoglobin, total proteins, and albumin levels were measured. Results revealed that plasma fibrinogen and serum haptoglobin levels were significantly increased in buffaloes with bronchiolitis and emphysema (P < 0.01), fibrinous bronchopneumonia (P < 0.05), and broncho-interstitial pneumonia (P < 0.01). In conclusion, serum haptoglobin and plasma fibrinogen levels are good indicators for the inflammatory conditions of the lungs in buffaloes.
    Comparative Clinical Pathology 07/2013; 23(4). DOI:10.1007/s00580-013-1696-6
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Bovine respiratory disease (BRD), which can cause substantial losses for feedlot operations, is often difficult to detect based solely on visual observations. The objectives of the current study were to determine a BRD case identification based on clinical and laboratory parameters and assess the value of feeding behavior for early detection of BRD. Auction-derived, mixed-breed beef steers (n = 213) with an average arrival weight of 294 kg were placed at a southern Alberta commercial feedlot equipped with an automated feed bunk monitoring system. Feeding behavior was recorded continuously (1-s intervals) for 5 wk after arrival and summarized into meals. Meals were defined as feeding events that were interrupted by less than 300 s non-feeding. Meal intake (g) and meal time (min) were further summarized into daily mean, minimum, maximum and sum, and together with frequency of meals per day, were fit into a discrete survival time analysis with a conditional log-log link. Feedlot staff visually evaluated (pen-checked) health status twice daily. Within 35 d after arrival, 76% (n = 165) of the steers had one or more clinical signs of BRD (reluctance to move, crusted nose, nasal or ocular discharge, drooped ears or head and gaunt appearance). While 41 blood samples could not be processed due to immediate freezing, for 124 of these steers, complete and differential blood cell count, total serum protein, plasma fibrinogen, serum concentration of haptoglobin (HP) and serum amyloid A (SAA) were determined. The disease definition for BRD was a rectal temperature ≥ 40.0°C, at least two clinical signs of BRD, and HP > 0.15 mg/mL. It was noteworthy that 94% of the 124 steers identified by the feedlot staff with clinical signs of BRD had HP > 0.15 mg/mL. An increase in mean meal intake, frequency and mean inter-meal interval time between meals was associated with a decreased hazard for developing BRD 7 d before visual identification (P < 0.001). Furthermore, increased mean mealtime, frequency and mean inter-meal interval time between meals were associated with a decreased BRD hazard up to 7 d before feedlot staff noticed clinical symptoms (P < 0.001). In conclusion, mean intake per meal as well as mean meal time and frequency of meals could be used to predict the hazard of BRD in feedlot cattle 7 d before visual detection and could be considered in commercial feedlot settings once a predictive algorithm has been developed.
    Journal of Animal Science 11/2014; 93(1). DOI:10.2527/jas.2014-8030 · 1.92 Impact Factor