Direct Visualization of Disulfide Bonds through Diselenide Proxies Using Se-77 NMR Spectroscopy

Institute for Molecular Bioscience, The University of Queensland, St. Lucia QLD 4072, Australia.
Angewandte Chemie International Edition (Impact Factor: 11.34). 11/2009; 48(49):9312-4. DOI: 10.1002/anie.200905206
Source: PubMed

ABSTRACT Se-ing is believing: Many proteins are cross-braced by disulfide bonds that frequently play key roles in protein structure, folding, and function. Unfortunately, the methods available for assignment of disulfide-bond connectivities in proteins are technically difficult and prone to misinterpretation. Now disulfide bond connectivities in native proteins can be visualized directly using 77Se NMR spectroscopy.

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