High-dynamic-range quantitative phase imaging with spectral domain phase microscopy.

Beckman Laser Institute and the Center for Biomedical Engineering, University of California, Irvine, Irvine, California 92612, USA.
Optics Letters (Impact Factor: 3.39). 11/2009; 34(21):3442-4. DOI: 10.1364/OL.34.003442
Source: PubMed

ABSTRACT Phase microscopy for high-dynamic-range quantitative phase-contrast imaging of a transparent phase object was demonstrated. Using a common path Fourier domain optical coherence tomography system, this technique is capable of displacement measurement with a sensitivity of 34 pm. The limitation of 2pi ambiguity restriction was overcome by the use of a phase retrieval approach performed in spectral domain. Two-dimensional quantitative phase imaging of human neonatal dermal keratinocyte cells was demonstrated to evaluate the performance of the system for cell imaging.

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