Laboratories of The Rockefeller Institute for Medical Research.
Journal of Experimental Medicine (Impact Factor: 13.91). 01/1922; 35(2):141-52.
Source: PubMed

ABSTRACT The massive agglutination observable in the shed blood of transfused rabbits, and associated not infrequently with sudden marked blood destruction, has a practical significance in connection with the untoward results of repeated transfusion from donors originally compatible; and it has special theoretical interest because the clumping of the cells is apparently an autoagglutination. To determine the actual source of the antibodies has been the object of the present work. The agglutination in its most marked form has been traced to isoantibodies elicited by the presence in the body of corpuscles originally found compatible; and the frequently associated, rapid blood destruction is doubtless of similar origin. Occasionally antibodies develop in the donor bloods during the period of transfusion, but they are so weak as to be negligible. There remain instances of what would seem to be true autoagglutination due to serum bodies induced by the transfusions as a by-product, so to speak, in the manufacture of isoagglutinins. The antigenic relationship between the red cells of different rabbits is so close that normal isoagglutinins became fixed in the cold upon their elaborator's own corpuscles. Agglutinins exist within the red cells of rabbits-as has been claimed by Klein. They are readily demonstrable in watery extracts of the dried corpuscles. Whether similar agglutinins ever exist within human cells remains to be determined. We have not found them in the normal corpuscles.

  • Source
    Blood 12/1951; 6(11):1021-33. · 9.78 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A pair of blood group factors, designated G and g, was identified in rabbits by serological means. These factors were found to be alleles, and one or the other or both of them were regularly present in the red cells of every one of a large number of mongrel and inbred rabbits. The factors were not demonstrable in other tissue cells or in the body fluids. They were capable of stimulating the formation of specific immune isoantibodies when repeatedly injected into appropriate rabbits. In most instance both agglutinating and coating antibodies to the G or g factors were present in a given antiserum. The coating antibodies-which did not act as true blocking antibodies-were detected by means of an antiglobulin test (Coombs test), or by means of specific cells modified by the action of trypsin. The antibodies were heat-stable and were active over a wide temperature range; and under suitable conditions, they proved to be potent hemolysins and also capable of fixing complement. The characteristics of the antigens and antibodies of the rabbit G-g system bear a striking resemblance to those of the Rh-Hr system of man.
    Journal of Experimental Medicine 02/1953; 97(1):33-49. DOI:10.1084/jem.97.1.33 · 13.91 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Four red cell antigen-antibody systems in the rabbit are described.Three of these systems, Z, Y and W, appear to be inherited as an allelomorphic series. The fourth is inherited independently.Some of the serological properties which allow these sera to be recognized are described and attention is drawn to a property of rabbit red cells, manifested by a variation in their susceptibility to agglutination by homologous antisera, which can complicate the separation of a mixture of antibodies in one serum.
    Journal of Hygiene 01/1956; 53(4):398-407. DOI:10.1017/S0022172400000905

Preview (2 Sources)

Available from