Article

Effects of estrogen on PMCA 2 and 4 in human fibroblast-like synovial cells and mouse macrophage-like cells.

Department of Oral and Maxillofacial Surgery, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
Endocrine Journal (impact factor: 2.03). 10/2009; 57(1):93-7. pp.93-7
Source: PubMed

ABSTRACT We investigated the possible roles of estrogen on plasma membrane Ca(2+)-ATPase (PMCA) in human fibroblast-like synovial cells (HFLS) and mouse macrophage-like cells (RAW 264.7). Western blots revealed the expression of PMCA 2 and 4 in both cells. In vitro treatments with 17beta-estradiol for 24 hours resulted in a concentration dependent decrease in PMCA expression. Moreover, Ca(2+)-ATPase specific activity was similarly decreased with estrogen treatments. However, treatments for 1 hour in the presence or absence of cycloheximide demonstrated non-significant effects. These results suggest that estrogen has a modulatory role on Ca(2+) homeostasis through decreasing PMCA expression and abating their activity.

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Keywords

1 hour
 
17beta-estradiol
 
abating
 
Ca(2+)-ATPase specific activity
 
concentration dependent decrease
 
cycloheximide
 
decreasing PMCA expression
 
human fibroblast-like synovial cells
 
modulatory role
 
mouse macrophage-like cells
 
plasma membrane Ca(2+)-ATPase
 
PMCA
 
PMCA 2
 
PMCA expression
 
possible roles