A conjugated linoleic acid-enriched beef diet attenuates lipopolysaccharide-induced inflammation in mice in part through PPARgamma-mediated suppression of toll-like receptor 4.
ABSTRACT Conjugated linoleic acid (CLA) is a PUFA found in beef and dairy products that has immunoregulatory properties. The level of CLA in beef can be enhanced by feeding cattle fresh grass rather than concentrates. This study determined the effect of feeding a high-CLA beef diet on inflammation in an in vivo model of septic shock. Mice were fed a high-CLA beef (4.3% total fatty acid composition) or low-CLA beef diet (0.84% total fatty acid composition) for 6 wk. Lipopolysaccharide (LPS; 3 microg) or sterile PBS was injected i.v. and serum was harvested 6 h after injection. Serum interleukin (IL)-1beta, IL-12p70, IL-12p40, and interferon-gamma concentrations were significantly reduced in response to the LPS challenge in the high-CLA beef diet group. Bone marrow-derived dendritic cells (BMDC) from the high-CLA beef diet group had significantly less IL-12 and more IL-10 in response to ex vivo LPS stimulation. Furthermore, toll-like receptor 4 (TLR4) and CD14 protein and mRNA expression on BMDC was significantly attenuated in the high-CLA compared with the low-CLA beef diet group. Complimentary in vitro experiments to determine the specificity of the effect showed that synthetic cis9, trans11-CLA suppressed surface expression of CD14 and TLR4 on BMDC. Treatment with the PPARgamma inhibitor GW9662 partially reversed TLR4 expression in immature BMDC. The results of this study demonstrate that feeding a diet enriched in high-beef CLA exerts profound antiinflammatory effects in vivo within the context of LPS-induced sepsis. In addition, downregulation of BMDC TLR4 is mediated through induction of PPARgamma.
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ABSTRACT: Modern nutritional science has been helped by a number of disciplines of a molecular nature including nutrigenomics, transcriptomics, proteomics and metabolomics. Together, these disciplines will make possible to find the nutritional footprint more appropriate for a given population, ethnic group, race, or more specifically to generate a personalized diet, according to the genetics and/or the phenotype of individuals. The expression of genes (transcriptomics) involves the synthesis of a few thousand of proteins (proteomics), which determine the phenotype of the individual producing a number of metabolites (metabolomics) that could be detected in different fluids of the body, but which also represent the work of a whole organization in homeostasis or outside it. No doubt, as the components of food affects the sequence transcriptomics, proteomics and metabolomics, the study of these disciplines is a research field that may allow us to have a comprehensive database that constitutes the fingerprint of nutrition. The objective of this article is to review some of the important aspects of how nutrients are involved in nutrigenomics.Revista Chilena de Nutricion 03/2012; 39(1):71-85.
- Cytokine 01/1996; · 2.87 Impact Factor
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ABSTRACT: Interest in probiotic bacteria, in the context of health and disease, is increasing and gathering scientific evidence, as is reflected by their growing utilization in food and pharma industry. As a consequence, a lot of research effort over the past few years has been dedicated to discern the molecular mechanisms responsible for their purported attributes. Remarkably, whereas the traditional probiotic concept assumes that bacteria must be alive during their administration in order to exert health promoting effects, evidence is being accumulated that supports defined bacterial secreted molecules and/or isolated surface components mediating attributed cross-talk dialogue between the host and the probiotic cells. Indeed, administration of the isolated bacterial-derived metabolites or molecules may be sufficient to promote the desired effects and may represent a promising safer alternative in inflammatory disorders. Here we summarise the current knowledge of molecular effectors of probiotic bacteria that have been involved in mediating their effects.This article is protected by copyright. All rights reserved.FEMS Microbiology Letters 08/2014; · 2.72 Impact Factor