Human metapneumovirus infection in a cohort of young asymptomatic subjects.

Section of Microbiology, Department of Pharmaco-Biology, University of Calabria, Rende (Cosenza), Italy.
The New Microbiologica: official journal of the Italian Society for Medical Virology (SIVIM) (Impact Factor: 1.6). 07/2009; 32(3):297-301.
Source: PubMed

ABSTRACT Human metapneumovirus (HMPV) is a recently described cause of respiratory infection. The purpose of this study was to to further the available information on the circulation of HMPV among young asymptomatic subjects. The epidemiology of HMPV was examined in a cohort of 73 university students during a winter season by using polymerase chain reaction. HPMV was detected in 3/73 (4.1%) asymptomatic subjects. Phylogenetic analysis has shown that circulating viruses belonged to different HMPV sublineages. Our findings indicate that asymptomatic carriage of HMPV might be a neglected source of viral transmission in the community.

1 Follower
  • [Show abstract] [Hide abstract]
    ABSTRACT: Exposure to the newly discovered human metapneumovirus (HMPV) during the first 2 years of life was studied by longitudinal serological analysis in 40 healthy children in southern Israel. The seropositivity rate decreased to a minimum by age 13 months and increased to 52% by age 24 months. Evidence of new infection was detected in 13%, 23%, and 55% of children by ages 7, 13, and 24 months, respectively. The high exposure rates suggest that HMPV may be an important cause of community-acquired respiratory-tract infections in young children.
    The Journal of Infectious Diseases 01/2004; 188(12):1865-7. DOI:10.1086/380100 · 5.78 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Human metapneumovirus (hMPV) is an emerging virus associated with acute respiratory tract infections (ARIs) in young children. To evaluate virologic and clinical features of hMPV infection during 2 consecutive winter-spring seasons. Nasal washes were obtained from children younger than 5 years of age hospitalized for ARI. Specimens were tested for hMPV by reverse transcription-polymerase chain reaction. The hMPV F gene amplification products were sequenced, and phylogenetic trees were constructed. A high incidence of hMPV infection (25.3%) was observed during the 2005-2006 winter-spring season, whereas a much lower rate of infection (4.7%) during the following season was found. Phylogenetic analysis revealed that, during the 2 seasons, 60.4% of the hMPV detected were A2a, 22.9% were A2b, 4.2% were B1, and 12.5% were B2. hMPV A1 strains were not detected in any tested specimen. Clinical diagnosis was bronchiolitis in 57.1%; pneumonia in 25%; and a upper respiratory tract illness in 17.8%. Bronchiolitis was more frequent in children less than 1 year of age (80%) than in children more than 1 year of age (30.8%) (P < 0.05). When hMPV was found frequently, the hMPV spread overlapped with that of respiratory syncytial virus (RSV) and hMPV/RSV coinfections were common events (19 of 39; 48.7%). hMPV/RSV-coinfected children developed pneumonia more frequently than hMPV-infected patients (57.9% versus 20%) but no differences in disease severity (gauged by duration of hospitalization and requirement of oxygen) were observed. These results provide further evidence of the importance of hMPV as a pathogen associated with ARI in young children. Involvement of hMPV/RSV coinfection in cases of pneumonia is suspected.
    The Pediatric Infectious Disease Journal 06/2008; 27(5):406-12. DOI:10.1097/INF.0b013e318162a164 · 3.14 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Human metapneumovirus (hMPV), a newly discovered paramyxovirus, has been associated with acute respiratory tract infections (ARIs) ranging from upper ARIs to severe bronchiolitis and pneumonia. Important questions remain on the contribution of hMPV to ARIs and its impact on public health. During the 2001-2002 season, we conducted a collaborative study with four provincial public health laboratories to study the prevalence of this new virus in the Canadian population. A total of 445 specimens were collected from patients of all age groups with ARIs and were tested for the presence of hMPV by reverse transcription-PCR. Of these, 66 (14.8%) tested positive for hMPV. Positive specimens were found in all age groups and in all four provinces studied. Virus activity peaked in February and March. The age range of the patients with hMPV infection was 2 months to 93 years (median age, 25 years), with similar numbers of females (35%) and males (41%). Thirty-three percent (n = 22) of hMPV-infected patients were hospitalized; of these, 27% (n = 6) had rhinitis and pneumonia, 23% (n = 5) had bronchiolitis, and 9% (n = 2) had bronchitis. The hospitalization rates were significantly higher among patients <5 years of age (P = 0.0005) and those >50 years of age (P = 0.0044) than among those 6 to 50 years of age. Phylogenetic analysis of the F gene showed that two hMPV genetic clusters were cocirculating in the 2001-2002 season, and comparison with earlier studies suggests a temporal evolutionary pattern of hMPV isolates. These results provide further evidence of the importance of hMPV in ARIs, particularly in young children and elderly individuals.
    Journal of Clinical Microbiology 10/2003; 41(10):4642-6. DOI:10.1128/JCM.41.10.4642-4646.2003 · 4.23 Impact Factor

Full-text (2 Sources)

Available from
May 17, 2014