Article

Rapid titrimetric and spectrophotometric methods for salbutamol sulphate in pharmaceuticals using N-bromosuccinimide.

Department of Chemistry, University of Mysore, Manasagangotri, Mysore-570 006, India.
Acta Pharmaceutica (Impact Factor: 1.16). 03/2007; 57(1):87-98. DOI: 10.2478/v10007-007-0007-7
Source: PubMed

ABSTRACT One titrimetric and two spectrophotometric methods which are simple, sensitive and rapid are described for the assay of salbutamol sulphate (SBS) in bulk drug and in tablet dosage forms using N-bromosuccinimide (NBS) and two dyes, rhodamine-B and methylene blue, as reagents. In titrimetry, aqueous solution of salbutamol sulphate is treated with a measured excess of NBS in acetic acid medium and after the oxidation of SBS is complete, the unreacted oxidant is determined iodometrically. Spectrophotometric methods entail addition of a known excess of NBS in acid medium followed by the determination of residual oxidant by reacting with a fixed amount of either rhodamine B and measuring the absorbance at 555 nm (method A) or methylene blue and measuring the absorbance at 665 nm (method B). In all methods, the amount of NBS reacting corresponds to the amount of SBS content. Titrimetric method is applicable over 1.74 x 10(-4) - 8.68 x 10(-4) mol L(-1) range and the reaction stoichiometry is found to be 1:6 (SBS:NBS). In spectrophotometric methods, the absorbance is found to increase linearly with the concentration of SBS, which is corroborated by the correlation of coefficients of 0.9993 and 0.9988 for method A and method B, respectively. The systems obey Beer's law for 0.25-1.75 microg mL(-1) (method A) and 0.5-5.0 microg mL(-1) (method B). The calculated apparent molar absorptivity values were found to be 2.10 x 10(5) and 6.16 x 10(4) L mol(-1) cm(-1), for method A and method B, respectively. The limits of detection and quantification are also reported for both spectrophotometric methods. Intra-day and inter-day precision and accuracy for the developed methods were evaluated. The methods were successfully applied to the assay of SBS in tablet and capsule formulations and the results were statistically compared with those of a reference method. No interference was observed from common tablet adjuvants. The accuracy and reliability of the methods were further ascertained by recovery experiments via the standard-addition technique.

0 Bookmarks
 · 
145 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple, precise, reproducible and accurate spectrofluorimetric method for estimation of Salbutamol sulphate (SAL) in bulk drug and various dosage forms has been developed. This method is based on formation of inclusion complex of SAL in β-cyclodextrin (BCD) which gives fluorescence at excitation wavelength of 279.6 nm and emission wavelength of 609.8 nm in water. Formation of inclusion complex of drug with BCD enhances fluorescence intensity of drug leads to increased sensitivity. The developed method was validated according to ICH guidelines with respect to accuracy, precision, linearity, limit of detection, limit of quantification. Linearity was observed in the range of 4-20 μg/ml with correlation coefficient of 0.9982. The simplicity of the method permitted rapid analysis suitable for routine control. The developed method was successfully applied for the estimation of SAL in different marketed dosage forms like tablets, syrup and aerosol.
    Pharmaceutical methods. 10/2010; 1(1):49-53.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A simple, rapid and accurate RP-HPLC method was developed for the determination of levosalbutamol in pure and tablet dosage form by RP-HPLC method using C 18 BDS column (Phenomenex, 250 x 4.6 mm, 5 μm) in isocratic mode. The mobile phase consisted of Acetonitrile and buffer in the ratio of 20:80 (v/v) was used and maintain the pH 3. The flow rate was maintained at 1 mL/min and the injection volume was 20 μL . Detection wavelength with UV detector at 276 nm and run time was kept 10 min. The retention time of levosalbutamol was 5.4 min. The method was linear over the concentration range 7-12 μg/ml. The recovery was found to be 100.44± 0.27%. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations.
    journal of applied pharmaceutical sciences. 06/2012; 02(2012):155-158.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A simple, rapid and accurate spectrophotometric method was developed for the determination of levosalbutamol in pure and rotacap dosage form . The proposed method is based on the principle that levosalbutamol exhibiting an absorption spectra of wavelength maxima 277 nm. This method has successfully used for the analysis of drug in marketed preparations in the range of 20-60 μg/ml with correlation coefficient of 0.998. The percentage recovery was found to be 99.25-101.14%. LOD and LOQ were found to be 1.81 and 5.48 μg/ml respectively. This method has been validated for linearity, accuracy and precision and found to be rapid, precise, accurate and economical and can be applied for routine estimation of levosalbutamol in solid dosage form. The validation of method was carried out utilizing ICH-guidelines.
    International Journal of Chemtech Applications. 08/2012; 1(1):16-20.

Full-text

Download
2 Downloads
Available from