A chemical platform for improved induction of human iPSCs.

Department of Chemistry, The Scripps Research Institute, La Jolla, California, USA.
Nature Methods (Impact Factor: 25.95). 11/2009; 6(11):805-8. DOI: 10.1038/nmeth.1393
Source: PubMed

ABSTRACT The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.

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Available from: Simon Hilcove, Jun 29, 2015
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