A chemical platform for improved induction of human iPSCs

Department of Chemistry, The Scripps Research Institute, La Jolla, California, USA.
Nature Methods (Impact Factor: 32.07). 11/2009; 6(11):805-8. DOI: 10.1038/nmeth.1393
Source: PubMed


The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.

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    • "We used the combination of LDN193189, SB431542, and TTNPB to initiate the reprogramming process by inhibiting glial signaling pathways and activating neuronal signaling pathways simultaneously. Tzv, an inhibitor of Rho-associated kinase (ROCK), promotes cell survival and improves reprogramming efficiency (Lin et al., 2009; Watanabe et al., 2007). Tzv was included throughout the 8 days of the reprogramming period. "
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    Cell stem cell 10/2015; DOI:10.1016/j.stem.2015.09.012 · 22.27 Impact Factor
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    • "PD and CH are used to convert human pluripotent stem cells to the naive state [4], [17]. Combination of SB and PD, or SB, PD, and sodium butyrate (NAB) can convert partially reprogrammed colonies to a fully reprogrammed state, thereby improving the efficiency of reprogramming [18], [19]. Moreover, epigenetic modifier NAB is more reliable and efficient than VPA in generation of human iPS cells and contributes to more efficient reprogramming [20], [21]. "
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    • "The path to reprogramming induced by Yamanaka factors involves multiple steps and various regulatory mechanisms [16], [17], [18], [19]. It is reported that some mircoRNAs (miRNAs) are involved in this path and hence can regulate the outcome of reprogramming. "
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