An experimental autoradiographic study of P32 tagged Candida albicans infection in rabbits

Source: OAI

ABSTRACT To study the pathogenesis of experimental candidiasis in animals, rabbits
given an intratraeheal injection of P32- labelled C. albicans in a suspension.
The organ immunity of the lung has been overcome by using a massive dose of fungus.
Dissemination of the fungus into all the distant organs was rapid and uniform. This can be achieved
only by a haematogenous route.
The autoradiography method used for (racing the isotope labelled C. aibicans showed that
apart from corroborating histopathoiogical lesions, and the culture of the fungus in tissues; the
autoradiography was positive during the last few days of the experiment, at which time all the
lesions were healed in the tissues and the fungus was negative by culture, and by histopathology,
This is an added method to identify Candida albians in tissues when conventional methods of
culture and histopathology of tissue have failed.



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