Article

The newt ortholog of CD59 is implicated in proximodistal identity during amphibian limb regeneration

da Silva, S.M. and Gates, P.B. and Brockes, J.P. (2002) The newt ortholog of CD59 is implicated in proximodistal identity during amphibian limb regeneration. Developmental Cell, 3 (4). pp. 547-555. ISSN 15345807 DOI:10266
Source: OAI

ABSTRACT The proximodistal identity of a newt limb regeneration blastema is respecified by exposure to retinoic acid, but its molecular basis is unclear. We identified from a differential screen the cDNA for Prod 1, a gene whose expression in normal and regenerating limbs is regulated by proximodistal location and retinoic acid: Prod 1 is the newt ortholog of CD59. Prod 1/CD59 was found to be located at the cell surface with a GPI anchor which is cleaved by PIPLC. A proximal newt limb blastema engulfs a distal blastema after juxtaposition in culture, and engulfment is specifically blocked by PIPLC, and by affinity-purified antibodies to two distinct Prod 1/CD59 peptides. Prod 1 is therefore a cell surface protein implicated in the local cell-cell interactions mediating positional identity.

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Keywords

affinity-purified antibodies
 
cDNA
 
cell surface protein
 
differential screen
 
distinct Prod 1/CD59 peptides
 
molecular basis
 
newt limb regeneration blastema
 
newt ortholog
 
PIPLC
 
Prod 1
 
proximal newt limb blastema engulfs
 
regenerating limbs
 
retinoic acid