Streptococcus alactolyticus is the dominating culturable lactic acid bacterium species in canine jejunum and feces of four fistulated dogs

FEMS microbiology letters. 2004. 230(1): 35-39 01/2006;
Source: OAI

ABSTRACT Canine intestinal lactic acid bacterium (LAB) population in four fistulated dogs was cultured and enumerated using MRS agar. LAB levels ranging from 1.4×106 to 1.5×107 CFU ml-1 were obtained in jejunal chyme. In the fecal samples 7.0×107 and 2.0×108 CFU g-1 were detected. Thirty randomly selected isolates growing in the highest sample dilutions were identified to species level using numerical analysis of 16 and 23 S rDNA RFLP patterns (ribotyping) and 16S rDNA sequence analysis. According to these results, Streptococcus alactolyticus was the dominant culturable LAB species in both faeces and jejunal chyme. In addition, Lactobacillus murinus and Lactobacillus reuteri were detected.

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    Applied Microbiology and Biotechnology 04/2013; · 3.69 Impact Factor
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    ABSTRACT: Although bifidobacteria and lactobacilli have been suggested beneficial for the host and are components of many probiotics and competitive exclusion mixtures, the knowledge on abundance, metabolic and probiotic characteristics in isolates from dogs and monkeys is still limited. The present study was aimed to isolate Bifidobacterium and Lactobacillus strains (faeces of 22 dogs and of 5 primates: Cebus apella, Eulemur fulvus, Erythrocebus patas, Macaca fascicularis, Papio hamadryas) with the MALDI-TOF identification system Lactobacillus murinus, Bifidobacterium animalis and Pediococcus acidilactici were more frequently isolated species in dogs while L. plantarum was isolated in several species of primates. Ten strains of 6 species were assayed for enzymatic activities (only L. reuteri strains showed no undesirable enzymatic activity), antimicrobial susceptibility (detected higher minimum inhibitory concentration levels for tetracycline and gentamicin), and inhibitory activity against 15 indicator bacteria. All strains inhibited Gram-negative indicators while lactobacilli showed larger inhibition zones than bifidobacteria. L. reuteri II/3b/a (isolate from Macaca fascicularis) showed the best antimicrobial properties. Resistance to bile (0.3 % w/v) was observed in all tested strains (no decrease of CFU/ml) whereas the decrease of 68.4 - 94.4 % (after 90 min exposition) and 78.4 - 99.9 % CFU/ml (after 180 min) depending on the strain was detected in the artificial gastric juice.
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