Poster FCBA spruceEmblingsFieldTrials TreebreedexLiverpool2009

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CANLET F, MAINE P, PREDHOMME JP, BRUNEAU G, REYMOND I, GASTINE F, BANET C, HARVENGT L
FCBA Technological Institute (formerly AFOCEL),Biotechnology lab
(luc.harvengt@fcba.fr), Domaine de l’Etançon, 77370 Nangis, France
A view of 10-15 year-old French Norway spruce
somatic embryo field trials (AFOCEL program)
Treebreedex, 2008, 21-23 April,
Liverpool (England)
PARTNER 26
INTRODUCTION - CONTEXT
FCBA is the french technological institute of the whole forest-wood-paper & furniture chain. It was funded in 2007
as the merger of AFOCEL and CTBA. AFOCEL was one of the pioneers of conifer Somatic Embryogenesis (SE)
during the 1980’s. Research was initially focussed on Norway spruce to understand the basic events associated
with SE and to set up methods, regarding particularly rejuvenation (SE from increasingly aged seedlings and tree).
Due to the drop of commercial demand for Norway spruce seeds and plants on the French market, the Norway
spruce SE program was terminated in 1999 as the same time as the breeding activity on this species, redirecting
efforts towards Pine. At this time, although hundreds of thousands of plants were produced on experimental
material, only a few field trials have been established. Here we present an overview of three of these trials.
Conclusion
1 - REMIREMONT test (Vosges)
2 - CHARREY/SAONE test (Côte d’or)
Trial design:
Planted April 1999
at 2.5 x 2 meters in
5 x 5 tree plots in
middle altitude
forest
Growth of unselected SE lines was compared to seedling material from the same genetic
provenances on the field over nine to twelve years. Although unselected clones were used, the
growth of SE plant material was good. In two out of three trials, SE material perform as well as the
corresponding seedlot (one year younger). The third trial, planted with material from earlier plant
Acknowledgement : The establishment of these filed tests was partly funded by EU through FP5 contract n°FAIR950873
1- REMIREMONT test: 3 years after plantation 1 – REMIREMONT test: 9 years after plantation
2 – CHARREY / SAONE test: 1 year after plantation 2 – CHARREY / SAONE test: 12 years after plantation
3 - NANGIS test: 1 year after plantation 3 - NANGIS test: 9 years after plantation
0
100
200
300
400
500
600
700
800
20911
(n=54)
A seedlot
(n=17)
B seedlot
(n=53)
C seedlot
(n=9)
1999
2000
2001
2002
2003
2004
2008
0
20
40
60
80
100
120
140
160
2000
2001
2002
2003
2004
2008
0
100
200
300
400
500
600
700
800
900
1000
1996
1997
1998
1999
2000
2002
2005
2008
0
10
20
30
40
50
60
70
80
90
100
Relative growth increase (%)
1997
1998
1999
2000
2002
2005
2008
WWW.FCBA.FR
The French technological institute
of the Forest-Wood-Paper &
Furniture Industry
Trial design: Planted April 1999
at 4 x 2 meters in 3 x 3 tree plots
Trial design:
Planted April 1996 at
3 x 2 meters in 4 x 4
tree plots in low
altitude farmland
YEAR
Shoot height (cm)
YEAR
Shoot height (cm)
Relative growth increase (%)
Fig. 3:
Fig. 3:Fig. 3:
Fig. 3: Re lative stem growth increase
Relative stem growth increaseRelative stem growth increase
Relative stem growth increase (means with 5% confidence limits)
Gérardmer Provenance
(Vosges –France) Chapois Provenance
(Jura –Fra nce)
Fig. 2:
Fig. 2:Fig. 2:
Fig. 2: Sh oot height
Shoot heightShoot height
Shoot height (means with 5% confidence limits)
0
50
100
150
200
250
Accroissement (%)
2000
2001
2003
2008
0
100
200
300
400
500
600
20911
(n=70) Q40
(n=58) Seedlings
(n=71) 541
(n=116) 611
(n=22) Seedlings
(n=62)
1999
2000
2001
2003
2008
YEAR
Shoot height (cm)
20911
(n=70) Q40
(n=58) Seedlings
(n=71) 541
(n=116) 611
(n=22) Seedlings
(n=62)
Gérardmer Provenance
(Vosges –France) Chapois Provenance
(Jura –Fra nce)
YEAR
Relative growth increase (%)
YEAR
YEAR
1
2
3
3- NANGIS test (Seine et Marne)
20911
(n=32)
30506
(n=21)
commercial
(n=30)
Excised
(n=32)
Unexcised
(n=32)
Ex vitro / in vitro germinated seedlingsEmblings
20911
(n=32)
30506
(n=21)
commercial
(n=30)
Excised
(n=32)
Unexcised
(n=32)
Ex vitro /in vitro germinated seedlingsEmblings
Figure 1 :Location of the three trials detailed below (red squares with numbers) and provenances
of the seedlots (blue arrows with letters : C : Chapois and Chablais, G: Gerardmer) used to initiate
SE lines and to produce control seedlings planted on the three trials.
Materials and Methods :
SE lines (named Q40, 20911 and 30506) were initiated from mature seeds of seedlots from 2 provenances. Plants for the trials were generated from lines
reactivated after thawing of cryopreserved material. SE plants were grown in the greenhouse for 2-6 months then transferred in the same containers for two
years in the Nangis nursery. Seedlings were germinated in the greenhouse in the same containers and transferred outside after 2 months for one year
before plantation in the field. Field plantation of seedlings and control seedlings from the same provenance were established in standard condition inside a
broader (multi-provenance) clonal field test. Blocks of 4 – 9 seedlings/emblings of a same provenance/SE line were established in a randomized design
standard condition plantation. Shoot height were mesured each one-five year(s). For the Charrey-sur-Saone trial, seedlings obtained from zygotic embryos
germinated in vitro with (« unexcised ») or without (« excised ») megagametophyte were used in addition to standard commercial seedlings. Exceptional
heat and drough t wave experienced during summer 2003 co uld explain some slowing down of plant growth this and next year(s).
G
C
Gérardmer Provenance
(Vosges – France)
Chablais Provenance
(Jura – France)
20911
(n=54)
A seedlot
(n=17)
B seedlot
(n=53)
C seedlot
(n=9)
Gérardmer Provenance
(Vosges – France)
Chablais Provenance
(Jura – France)
batches, shows lower performance of SE material. Complementary experiments,
including additional field trials, demonstrated that this lower performance was due
to suboptimal ex vitro management. Indeed, the slower initial growth of emblings
necessitated adaptation of plant management to get good performance.