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Available from: Pilar García Rebollar, Aug 10, 2015
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    • "Even if Tris-buffers are commonly used to dilute rabbit fresh semen (Viudes de Castro, 1996 and 1997; Castellini et al., 2000; Roca et al,. 2000; Lavara et al., 2005) commercially available porcine extenders have been used without decreasing the seminal parameters quality (Nagy et al., 2002; Rosato et al., 2006; Piglet® and MIII®, respectively). Since these extenders are cheaper and easier to prepare than those based on tris buffers, their use in farms would be very useful. "
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    ABSTRACT: An improvement in reproduction management has been associated to the wide use of artificial insemination in rabbits. The use of frozen semen in this species is greatly limited due to its low fertility rates. Moreover, in politocous species, prolificacy is a very important trait and it is particularly affected when semen is frozen. Since gelatine addition to fresh semen extenders improves and prolongs motility and viability sperm parameters, its use in semen freezing could enhance sperm efficiency after thawing. The aim of this study was to assess the effects of gelatine addition to freezing extender using a commercially available extender (MIII) and a tris-based extender (TGC). Four experimental extenders were used: TGC extender with gelatine, the same without gelatine and MIII extender with and without gelatine. In order to evaluate seminal parameters, motility was assessed using the Computer Assisted Sperm Analysis, while viability rate was calculated using flow cytometry. To evaluate in vivo frozen-thawed semen, 273 females were inseminated. No significant difference was found among the four freezing extenders when motility, viability fertility or prolificacy were considered. Motility rates ranged between 22.4% and 35.1%, while viability varied between 22.5 and 30.9%. Fertility rates oscillated from 80.8% to 86.2% and the total born ranged from 7.9 to 8.5. While in vitro results were lower than those obtained in other studies, in vivo results were quite favourable. In conclusion, gelatine addition did not improve sperm motility and viability after thawing nor fertility and prolificacy after insemination with frozen-thawed semen, using MIII or TCG extender.
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    ABSTRACT: Failures in fertilization or embryogenesis have been shown to be partly the result of poor semen quality. When AI is practiced, fertilization rate depends on the number and quality of spermatozoa in the insemination dose around the time of application. Individual variation in the male effect on fertility (success or failure to conceive; Fert) and prolificacy (total number of kids born per litter; TB) could also depend on these factors, and it could be better observed under limited conditions of AI, such as decreased sperm concentration, small or null preselection of ejaculates for any semen quality trait, or a long storage period of the AI doses. The aim of this research was to determine if an interaction existed between male genotype and the AI conditions for male effects on Fert and TB after AI was performed under different conditions. Fertility and TB were assumed to be different traits and were analyzed in 2 sets of independent analyses. In the first step, the different conditions were determined uniquely by the sperm dosage. Artificial insemination was performed at 10 and 40 × 10(6) spermatozoa/mL. In the second step, the different conditions were determined by all the factors involved in the AI process as a whole (conditions and duration of the storage period of the dose, genetic type of the female, and environmental conditions on the farm). Data from AI from the former experiment were analyzed with data from AI performed under different conditions. Threshold and linear 2-trait models were assumed for Fert and TB, respectively. The sperm dosage had a clear effect on Fert and TB, which favored the greater dosage (+0.13% and +1.25 kids born, respectively). Prolificacy was more sensitive to sperm reduction than was fertility. Male heritabilities for Fert were 0.09 for both sperm dosages, and were 0.08 and 0.06 for male TB with a smaller and larger sperm dosage, respectively. No genotype × sperm dosage interaction was found. Therefore, the same response to selection to improve male Fert and TB could be achieved at any sperm concentration. However, an interaction between male genotype and the AI conditions as a whole seemed to exist, indicating that the AI conditions for selection for Fert and TB could be modified to maximize genetic progress. Consequently, the optimization of a breeding program for male Fert and TB under a given set of semen utilization conditions is achievable.
    Journal of Animal Science 11/2010; 88(11):3475-85. DOI:10.2527/jas.2009-2773 · 1.92 Impact Factor
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    ABSTRACT: Two experiments were carried out to examine in vitro quality and in vivo fertility of rabbit semen diluted in ultra-high temperature (UHT) skim milk. In the first experiment, pooled ejaculates of 10 adult rabbits were divided in three aliquots. Each aliquot was diluted in saline solution, TrisC or UHTm extender and kept at room temperature for 24 h. Sperm quality assessment was performed during all the incubation periods. In the second experiment, 27 adult rabbit does were inseminated with semen incubated for 5 h. Embryo recovery was performed 96 h after insemination. Results showed that treatments diluted in UHTm registered the highest values of spermatozoon with total motility, intact and functional plasma membrane and greater number of embryos recovered in rabbit does. We conclude that UHT skim milk would be a good extender for improved intra-uterine insemination in rabbits and to keep sperm cells for several hours at room temperature. © 2015 Blackwell Verlag GmbH.
    Andrologia 06/2015; DOI:10.1111/and.12445 · 1.17 Impact Factor