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  • Annual Review of Entomology 01/1995; 40(1):511-534. DOI:10.1146/annurev.ento.40.1.511 · 13.73 Impact Factor
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    ABSTRACT: The whitefly Bemisia tabaci is the only vector of the tomato yellow leaf curl geminivirus (TYLCV). The insect transmits the virus in a persistent-circulative manner. TYLCV DNA was detected by polymerase chain reaction and by Southern blot hybridization in progeny (eggs, first and second instars, adults) of single viruliferous whiteflies that developed on eggplant or on cotton (two TYLCV nonhost plants). Furthermore, TYLCV DNA was present in the progeny of insects that had acquired the virus through the egg. The adult progeny of the viruliferous insects and their own progeny were able to infect tomato test plants, producing typical disease symptoms. Ovaries and maturing eggs of viruliferous insects contained viral DNA, as did eggs laid by viruliferous insects maintained on an artificial diet Eggs laid by nonviruliferous whiteflies on cotton plants previously caged with viruliferous insects did not acquire viral DNA from the plant. Hence, TYLCV can be transmitted through the egg for at least two generations. In the absence of an available plant host, the whitefly may serve as a reservoir of the virus between growing seasons.
    Virology 02/1998; 240(2):295-303. DOI:10.1006/viro.1997.8937 · 3.32 Impact Factor
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    ABSTRACT: The yellow leaf curl disease of tomato is caused by a complex of virus species, two of which, tomato yellow leaf curl virus (TYLCV)-Sar and TYLCV-Is, are involved in epidemics of southern Spain. Plants of Mercurialis ambigua and Solanum luteum showing abnormal upward leaf curling and leaf distortion collected in the vicinity of tomato crops were found to be naturally infected with TYLCV-Is and TYLCV-Sar, respectively. These weed species, as well as Datura stramonium and S. nigrum, which had also been found to be naturally infected by TYLCVs in the same region in previous studies, were tested for susceptibility to TYLCV-Sar or TYLCV-Is by Agrobacterium tumefaciens-mediated and by Bemisia tabaci inoculation. Results indicated that both TYLCV-Sar and TYLCV-Is were able to infect D. stramonium and M. ambigua, whereas only TYLCV-Sar infected S. nigrum and S. luteum. Implications for the epidemiology of TYLCV are discussed. This is the first report of M.ambigua and S. luteum as hosts of TYLCV.
    European Journal of Plant Pathology 04/2000; 106(4):391-394. DOI:10.1023/A:1008758622582 · 1.49 Impact Factor