Article

Hepatoprotective effects of salidroside on fulminant hepatic failure induced by D-galactosamine and lipopolysaccharide in mice.

Key Laboratory for Natural Resource of Changbai Mountain & Functional Molecules, Ministry of Education, College of Pharmacy, Yanbian University, Jilin Province, China.
The Journal of pharmacy and pharmacology 10/2009; 61(10):1375-82. DOI:10.1211/jpp/61.10.0015 pp.1375-82
Source: PubMed

ABSTRACT The aim was to investigate the protective effect of salidroside isolated from Rhodiola sachalinensis A. Bor. (Crassulaceae) on D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure.
Hepatotoxicity was induced by an intraperitoneal injection of D-galactosamine (700 mg/kg) and lipopolysaccharide (10 mug/kg); salidroside (20, 50 and 100 mg/kg) was administered intraperitoneally 1 h before induction of hepatoxicity. Liver injury was assessed biochemically and histologically.
Salidroside attenuated the induced acute increase in serum aspartate aminotransferase and alanine aminotransferase activities, and levels of tumour necrosis factor-alpha levels and serum nitric oxide. It restored depleted hepatic glutathione, superoxide dismutase, catalase and glutathione peroxidase activities, decreased malondialdehyde levels and considerably reduced histopathological changes. Histopathological, immunohistochemical and Western blot analyses also demonstrated that salidroside could reduce the appearance of necrotic regions and expression of caspase-3 and hypoxia-inducible factor-1alpha in liver tissue.
Salidroside protected liver tissue from the oxidative stress elicited by D-galactosamine and lipopolysaccharide. The hepatoprotective mechanism of salidroside appear to be related to antioxidant activity and inhibition of hypoxia-inducible factor-1alpha.

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Keywords

alanine aminotransferase activities
 
D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure
 
glutathione peroxidase activities
 
Histopathological
 
histopathological changes
 
hypoxia-inducible factor-1alpha
 
induction
 
inhibition
 
intraperitoneally 1 h
 
lipopolysaccharide
 
Liver injury
 
liver tissue
 
oxidative stress elicited
 
protective effect
 
Rhodiola sachalinensis A. Bor
 
serum aspartate aminotransferase
 
serum nitric oxide
 
superoxide dismutase
 
tumour necrosis factor-alpha levels
 
Western blot analyses