Depressed Antioxidant Status in Pregnant Women on Iron Supplements: Pathologic and Clinical Correlates

Department of Chemical Pathology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Biological trace element research (Impact Factor: 1.75). 10/2009; 136(2):157-70. DOI: 10.1007/s12011-009-8534-3
Source: PubMed


Iron (Fe) remains a commonly prescribed supplement in pregnancy. Its possible pathologic potential is either uncommonly considered or ignored. We determined the antioxidant status in pregnant women with and without Fe supplements. Fifty-eight apparently healthy pregnant women on Fe supplements were selected for the study from the antenatal clinic of the University College Hospital, Ibadan, Nigeria. Fifty-five aged matched pregnant women who were not on Fe from various parishes of the Christ Apostolic Church, Ibadan (non-drug using Christian sect) were randomly selected as controls. Both groups were classified according to the trimesters of pregnancy. The gestational age in both pregnant women on Fe supplements and non-supplement pregnant women was similar. Fruit and vegetables consumption was higher in the supplement than in the non-supplement group (57.2% vs. 37.3%). Anthropometric indices, weight, height, and BMI, were also similar. But while the weight of the Fe supplement group decreased by nearly 3% in the third trimester, it increased by over 10% (p < 0.00) in the non-supplement group in the same period. Serum Fe level was significantly higher in the supplement than the non-supplement group (p < 0.001). In contrast, the levels of the antioxidants, ascorbic acid, copper (Cu), zinc (Zn), and bilirubin were all significantly decreased (p < 0.05, p < 0.001, p < 0.05, and p < 0.05, respectively). Uric acid level though also lower in the supplement group did not reach statistical significance (p > 0.05), while vitamin E was similar in both groups. There was relative stability of all antioxidants except uric acid, which declined from the first to the last trimester in the non-supplement group. The significantly higher Fe level in the second trimester was sustained in the third trimester though to a lesser degree (p < 0.05) and associated with significant decreases in the following antioxidant levels in the supplement group, ascorbic acid, bilirubin, Cu, and Zn (p < 0.02, p < 0.02, p < 0.02, and p < 0.001, respectively). Uric acid and vitamin E though lower in the supplement group were not significantly different. Remarkably, percentage changes between the first and third trimesters revealed that serum Fe increased by over 116% in the Fe supplement group, while it only increased by over 50% in the non-supplement group. This was associated with 23.50% decrease in ascorbate level (p < 0.003) in the supplement group, while it decreased by only 3.70% in the non-supplement group (p > 0.05). Again vitamin E decreased by 17.22% in the supplement group, while it decreased by only 7.30% in the non-supplement group during the period. Uric acid and bilirubin levels decreased by similar proportions during the period, while Zn decreased by 18.55% in the supplement group and by 14.86% in the non-supplement group. In contrast Cu increased by 7.20% in the supplement group, while it increased by only 2.96 in the non-supplement group. Additionally, all the antioxidants in the supplement group except vitamin E, viz, ascorbic acid, bilirubin, Cu, uric acid, and Zn, were significantly inversely correlated with serum Fe level (r - 0.299, p < 0.05, r - 0.278, p < 0.05, r - 0.383, p < 0.05, and r - 0.0369, p < 0.05). These data imply markedly depressed antioxidant status in the Fe supplement pregnant group with attendant oxidative stress (most probably pro-oxidant Fe-induced). This is associated with molecular and cellular damage as well as a number of pathologic and clinical correlates that underlie the exacerbation of morbidity and mortality in maternal and child populations, particularly in the developing countries. This appears to call for serious caution and prior evaluation of antioxidant and Fe status and during the use of Fe supplements in pregnancy for monitoring and prognostic purposes and to avert or ameliorate oxidative stress-induced pathologies in maternal and fetal systems.

3 Reads
  • Source
    • "The contribution of dietary antioxidants to this overall increase cannot be ruled out. Specific endogenous antioxidants, such as ascorbic acid, vitamin E and zinc, have been analyzed in all three trimesters of normal pregnancies and shown to be inversely related to gestational age, decreasing gradually as pregnancy proceeds toward term (Patil et al., 2007; Anetor et al., 2010). Smoking in mothers has a deleterious effect on both maternal and fetal oxidative and antioxidant status, increasing free radical damage and decreasing antioxidant potential (Chelchowska et al., 2011). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Discussion continues as to whether de novo hypertension in pregnancy with significant proteinuria (pre-eclampsia; PE) and non-proteinuric new hypertension (gestational hypertension; GH) are parts of the same disease spectrum or represent different conditions. Non-pregnant hypertension, pregnancy and PE are all associated with oxidative stress. We have established a 6 weeks post-partum clinic for women who experienced a hypertensive pregnancy. We hypothesized that PE and GH could be distinguished by markers of oxidative stress; thiobarbituric acid reactive substances (TBARS) and antioxidants (ferric ion reducing ability of plasma; FRAP). Since the severity of PE and GH is greater pre-term, we also compared pre-term and term disease. Fifty-eight women had term PE, 23 pre-term PE, 60 had term GH and 6 pre-term GH, 11 pre-existing (essential) hypertension (EH) without PE. Limited data were available from normotensive pregnancies (n=7) and non-pregnant controls (n=14). There were no differences in postpartum TBARS or FRAP between hypertensive states; TBARS (P=0.001) and FRAP (P=0.009) were lower in plasma of non-pregnant controls compared to recently-pregnant women. Interestingly FRAP was higher in preterm than term GH (P=0.013). In PE and GH, TBARS correlated with low density lipoprotein (LDL)-cholesterol (P=0.036); this association strengthened with inclusion of EH ((P=0.011). The 10 year Framingham index for cardiovascular risk was positively associated with TBARS (P=0.003).Oxidative stress profiles do not differ between hypertensive states but appear to distinguish between recently-pregnant and non-pregnant states. This suggests that pregnancy may alter vascular integrity with changes remaining 6 weeks postpartum. LDL-cholesterol is a known determinant of oxidative stress in cardiovascular disease and we have shown this association to be present in hypertensive pregnancy further emphasizing that such a pregnancy may be revealing a pre-existing cardiovascular risk.
    Frontiers in Physiology 08/2014; 5:310. DOI:10.3389/fphys.2014.00310 · 3.53 Impact Factor
  • Heart (British Cardiac Society) 07/1964; 26(4):524-527. DOI:10.1136/hrt.26.4.524 · 5.60 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Purpose – Iron deficiency anaemia (IDA) is a common problem in pregnancy and may influence the health of both mother and child. The purpose of this paper is to discuss current prevention and treatment strategies for pregnancy IDA and describe alternative methods, such as food-based approaches that may be another way forward. Design/methodology/approach – A literature review was conducted to locate and summarise up-to-date published studies within the field. Findings – There is good evidence that iron supplements may be used to prevent and treat IDA in pregnancy. However, there is much debate about “the optimal” dose to recommend. High doses may contribute to oxidative stress and cause gastrointestinal symptoms while there still remain problems with compliance, even for lower dose iron supplements. Encouraging the daily consumption of functional foods containing suitable levels of iron may therefore be an alternative way to improve pregnancy iron status. Research limitations/implications – There is a wealth of important studying the health implications of iron supplements in pregnancy. More work is needed to establish whether functional foods containing iron could be an alternative way to help improve iron status. Originality/value – Most papers and research focus on supplement use as a preventative measure and treatment for IDA; few discuss other approaches.
    Nutrition & Food Science 11/2010; 40(6):598-607. DOI:10.1108/00346651011090400
Show more