Development and Characterization of New Microsatellite Markers for the Oyster Mushroom (Pleurotus ostreatus)

National Agrobiodiversity Center, National Academy of Agricultural Science, RDA, Suwon 441-707, Korea.
Journal of Microbiology and Biotechnology (Impact Factor: 1.53). 09/2009; 19(9):851-7. DOI: 10.4014/jmb.0811.604
Source: PubMed


We developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (Pleurotus ostreatus). In total, 169 alleles were identified with an average of 4.7 alleles per locus. Values for observed (HO) and expected (HE) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. Nineteen loci deviated from Hardy-Weinberg equilibrium. Significant (P<0.05) excess heterozygosity was observed at nine loci. Linkage disequilibrium (LD) was significant (P<0.05) between pairs of locus alleles. Cluster analysis revealed that five species of genus Pleurotus made a distinct group, and the individual cultivars were grouped into major five groups from G-1 to G-5. The diverse cultivars of P. ostreatus were discriminated and the other four species revealed a different section in the UPGMA tree. These microsatellite markers proved to be very useful tools for genetic studies, including assessment of the diversity and population structure of P. ostreatus.


Available from: Won-Sik Kong
  • Source
    • "This will give farmers an opportunity not to arrive at an erroneous conclusion by recognizing the targeted species as an incorrect species [39, 40]. As noted earlier, a number of molecular based markers have been hitherto employed to investigate phylogenetic relationship and taxonomic hierarchy of the edible mushrooms, particularly Pleurotus genus [9, 41]. However, many such studies on fungal strain identification have been performed by common morphological characteristics [5, 11] which were unreliable and misleading [42, 43]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Identification of edible mushrooms particularly Pleurotus genus has been restricted due to various obstacles. The present study attempted to use the combination of two variable regions of IGS1 and ITS for classifying the economically cultivated Pleurotus species. Integration of the two regions proved a high ability that not only could clearly distinguish the species but also served sufficient intraspecies variation. Phylogenetic tree (IGS1 + ITS) showed seven distinct clades, each clade belonging to a separate species group. Moreover, the species differentiation was tested by AMOVA and the results were reconfirmed by presenting appropriate amounts of divergence (91.82% among and 8.18% within the species). In spite of achieving a proper classification of species by combination of IGS1 and ITS sequences, the phylogenetic tree showed the misclassification of the species of P. nebrodensis and P. eryngii var. ferulae with other strains of P. eryngii. However, the constructed median joining (MJ) network could not only differentiate between these species but also offer a profound perception of the species' evolutionary process. Eventually, due to the sufficient variation among and within species, distinct sequences, simple amplification, and location between ideal conserved ribosomal genes, the integration of IGS1 and ITS sequences is recommended as a desirable DNA barcode.
    The Scientific World Journal 01/2014; 2014(10):793414. DOI:10.1155/2014/793414 · 1.73 Impact Factor
  • Source
    • "Edible species of mushroom are low in calories, fats, sodium, carbohydrates and cholesterol, whereas, rich in proteins, minerals, vitamins and fibres (Nasim et al. 2001). Besides nutritional importance genus Pleurotus is also known for its medicinal value (Kothe 2001), paper pulp bleeching, cosmetics and industrial use (Kyung-Ho Ma et al. 2009; Sigoillot et al. 2005). However, Pleurotus is ranked second to the button mushroom (Kues and Liu 2000), but low yield level, inconsistency in flush appearance, texture, color and taste affects its adoption as the favorite mushroom for cultivation. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Existence of variability in morphological traits and growth rate of mycelium of homokaryotic single basidiospores can be exploited for the development of inter-strainal hybrids. We isolated 182 single basidiospores from mushroom bodies of P. sajor-caju, P. florida, P. eous and one wild relative of Pleurotus called Hypsizygus ulmaris. The single spores were isolated using a new technique that is less prone to contamination and more efficient than the common techniques used by earlier workers. All the isolates showed a varied range of cultural morphology. Mating types of all the isolates within the species were identified on the basis of hyphal fusion via anastomosis with the tester strains. Four compatible pairs of isolates with well prominent tuft in the contact zone were selected for dikaryon isolation. Dikaryons were used for spawn preparation and mushroom cultivation. The dikaryotic isolates with their replicates were evaluated for spawn run period, yield and biological efficiency. 42 isolates (10 di- and 32 mono-karyotic isolates) were analyzed with RAPD genetic markers. Phenotypic characters and mating types of all the 42 isolates analyzed genetically were correlated with their genetic polymorphism data. The isolates showed very large diversity both at the phenotypic and the genotypic level. Available phenotypic and genotypic data can further help in the selection of monosporous isolates for developing inter-strainal hybrids which can lead to better prospects for genetic improvement in different species of Pleurotus. KeywordsSingle spore– Pleurotus spp.–Dikaryon–Homokaryotic isolates–Random amplified polymorphic DNA (RAPD)
    World Journal of Microbiology and Biotechnology 01/2011; 27(1):1-9. DOI:10.1007/s11274-010-0419-2 · 1.78 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, microsatellite markers were utilized to reveal the genetic diversity of 56 strains of Lentinula edodes grown in China. A total of 224 DNA bands were detected through 25 primer pairs, of which, 223 bands (99.6%) were polymorphic between two or more strains. The variation in SSR (Simple Sequence Repeat) DNA band patterns and average genetic similarity among the wild strains of L. edodes obtained from the same region uncovered a vast genetic diversity in the natural germplasm found in China. Compared with L. edodes strains from other areas, the genetic diversity of those from the Yunnan Plateau, Hengduanshan mountains, Taiwan, and south China was significantly greater. Based on cluster analysis and principal coordinate analysis, the results indicated that all L. edodes strains could be divided into three major groups. These results effectively displayed the differences between the strains from North and South China, and those from the same or adjoining regions could cluster preferentially into small groups in most cases, suggesting the positive correlation between the cluster results and the geographical origin for the natural germplasm of Chinese L. edodes. To our knowledge, this is a pioneering report on the utilization of the SSR marker technique in analyzing L. edodes’ genetic diversity. KeywordsShiitake mushroom-Microsatellite-DNA polymorphism-UPGMA-PCoA
    World Journal of Microbiology and Biotechnology 03/2009; 26(3):527-536. DOI:10.1007/s11274-009-0202-4 · 1.78 Impact Factor
Show more