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The Tor and PKA signaling pathways independently target the Atg1/Atg13 protein kinase complex to control autophagy. Proc Natl Acad Sci USA

Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
Proceedings of the National Academy of Sciences (Impact Factor: 9.81). 10/2009; 106(40):17049-54. DOI: 10.1073/pnas.0903316106
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ABSTRACT Macroautophagy (or autophagy) is a conserved degradative pathway that has been implicated in a number of biological processes, including organismal aging, innate immunity, and the progression of human cancers. This pathway was initially identified as a cellular response to nutrient deprivation and is essential for cell survival during these periods of starvation. Autophagy is highly regulated and is under the control of a number of signaling pathways, including the Tor pathway, that coordinate cell growth with nutrient availability. These pathways appear to target a complex of proteins that contains the Atg1 protein kinase. The data here show that autophagy in Saccharomyces cerevisiae is also controlled by the cAMP-dependent protein kinase (PKA) pathway. Elevated levels of PKA activity inhibited autophagy and inactivation of the PKA pathway was sufficient to induce a robust autophagy response. We show that in addition to Atg1, PKA directly phosphorylates Atg13, a conserved regulator of Atg1 kinase activity. This phosphorylation regulates Atg13 localization to the preautophagosomal structure, the nucleation site from which autophagy pathway transport intermediates are formed. Atg13 is also phosphorylated in a Tor-dependent manner, but these modifications appear to occur at positions distinct from the PKA phosphorylation sites identified here. In all, our data indicate that the PKA and Tor pathways function independently to control autophagy in S. cerevisiae, and that the Atg1/Atg13 kinase complex is a key site of signal integration within this degradative pathway.

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    • "Consequently, in addition to p62 and the ubiquitination machinery, cAMP signalling could be misregulated in these cases, leading to hyperphosphorylation of p62 at Ser24, suppression of homopolymerisation and hence a reduction in autophagy . Interestingly, recent data from the yeast S. cerevisiae shows that increased cAMP/PKA activity blocks autophagy [39], and that inactivation of the cAMP pathway induces autophagy [40]. However, yeast do not express p62 or homologues thereof, and the autophagyregulating protein Atg13 was identified as the responsible PKA substrate in this case. "
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    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 08/2014; 1843(11). DOI:10.1016/j.bbamcr.2014.07.021 · 5.30 Impact Factor
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    • "The inhibition of MTOR signaling by glucagon was later shown to proceed through a mechanism dependent on protein kinase A ( Kimball et al . 2004 ) , which stimulates autophagy ( Mavrakis et al . 2006 ) . It must be pointed out that , in contrast to mammalian cells , activation of protein kinase A in yeast cells inhibits autophagy ( Stephan et al . 2009 ) . In this context , it is important to stress that the function of cAMP in yeast , in contrast to that in mamma - lian cells , is to stimulate cell growth ( Thevelein and de Winde 1999 ) ."
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    Amino Acids 06/2014; DOI:10.1007/s00726-014-1765-4 · 3.65 Impact Factor
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    • "Target of rapamycin protein kinase complex 1 and Sch9 function in networks to regulate cellular processes, and one network link is with (PKA) which, like TORC1 and Sch9, plays roles in nutrient and stress sensing and CLS (Fig. 1A) (Soulard et al., 2010; De Virgilio, 2012; Longo et al., 2012). To determine whether the combination drug treatment lowers PKA activity, we examined Atg13 phosphorylation , as the phosphorylated form of this protein maintains autophagic flux at a low basal level in log-phase cells (Stephan et al., 2009). Combination drug treatment causes a decrease in PKA-mediated phosphorylation of Atg13 compared with single or no drug treatment (Fig. 4D, top panel). "
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    Aging cell 04/2013; 12(4). DOI:10.1111/acel.12090 · 5.94 Impact Factor
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