Article

Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.

Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
Journal of Bioscience and Bioengineering (Impact Factor: 1.74). 11/2009; 108(5):446-9. DOI:10.1016/j.jbiosc.2009.05.005
Source: PubMed

ABSTRACT We established a novel method, insertional chromatin immunoprecipitation (iChIP), for isolation of specific genomic regions. In iChIP, specific genomic domains are immunoprecipitated with antibody against a tag, which is fused to the DNA-binding domain of an exogenous DNA-binding protein, whose recognition sequence is inserted into the genomic domains of interest. The iChIP method will be a useful tool for dissecting chromatin structure of genomic region of interest.

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