Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions

Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.
Journal of Bioscience and Bioengineering (Impact Factor: 1.79). 11/2009; 108(5):446-9. DOI: 10.1016/j.jbiosc.2009.05.005
Source: PubMed

ABSTRACT We established a novel method, insertional chromatin immunoprecipitation (iChIP), for isolation of specific genomic regions. In iChIP, specific genomic domains are immunoprecipitated with antibody against a tag, which is fused to the DNA-binding domain of an exogenous DNA-binding protein, whose recognition sequence is inserted into the genomic domains of interest. The iChIP method will be a useful tool for dissecting chromatin structure of genomic region of interest.

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Available from: Hodaka Fujii, Aug 24, 2015
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    • "It would be interesting future issues to investigate the functions of newly identified chromatin-binding proteins. We recently developed iChIP, which is a method to biochemically isolate genomic regions of interest [9] [11]. iChIP has been successfully used to directly identify proteins, RNA and DNA bound to specific genomic regions in vivo [10] [30]. "
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