Deoxyribonucleic acid ploidy in endometrial carcinoma: A reproducible and valid prognostic marker in a routine diagnostic setting

Institute of Clinical Medicine, University of Bergen, and Department of Obstetrics and Gynecology, Haukeland University Hospital, Bergen, Norway.
American journal of obstetrics and gynecology (Impact Factor: 4.7). 10/2009; 201(6):603.e1-7. DOI: 10.1016/j.ajog.2009.07.029
Source: PubMed


The objective of the study was to investigate the prognostic impact of deoxyribonucleic acid (DNA) ploidy in endometrial carcinoma in a routine diagnostic series as compared with a research series.
We studied a population-based series of 363 endometrial carcinomas prospectively collected, with long and complete follow-up. The prognostic value of DNA ploidy was investigated in a routine diagnostic series (n=262) and compared with the results from a previous research series (n=101).
The proportion of DNA aneuploid tumors was 21% in the research series and 25% in the routine diagnostic series (P=NS). In both series, DNA aneuploidy was significantly correlated to higher age at diagnosis, nonendometrioid subtype, and high histologic grade. Patients with DNA aneuploid tumors had significantly poorer survival, adjusted for established clinicopathologic prognostic factors.
DNA ploidy estimation in endometrial carcinoma adds independent prognostic information in a routine diagnostic setting.

5 Reads
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This paper introduces an efficient technique for designing a parameterized family of one- and multi-dimensional filters by regarding it as a single multi-dimensional filter. The new design technique is very fast since it reduces the design of a large set of filters to the design of a complex or real one-dimensional filter which is then mapped to produce the desired set of filters. It has the additional advantage that the one- and multi-dimensional filters in the set can be designed and realized efficiently without having to explicitly compute the coefficients of the separate filters. Among the types of filters which can be designed with this technique are seismic migration filters and linear-phase filters
    Acoustics, Speech, and Signal Processing, 1996. ICASSP-96. Conference Proceedings., 1996 IEEE International Conference on; 06/1996
  • [Show abstract] [Hide abstract]
    ABSTRACT: DNA ploidy has been reported to be a prognostic marker for patients with endometrial carcinoma. In this study, DNA ploidy and histologic heterogeneity were evaluated by comparing curettage and hysterectomy specimens in 99 consecutive patients diagnosed with endometrial carcinoma. High-resolution DNA ploidy image analysis and review of histologic specimens were performed. The histologic subtypes were identical in 77 (78%) and differed in 22 (22%) cases. The DNA ploidy results were concordant in the curettage and hysterectomy specimens in 72 (72.7%) and discordant in 27 (27.3%) cases. Histologic heterogeneity was significantly associated with DNA ploidy heterogeneity (P=0.03). On the basis of histologic heterogeneity, DNA ploidy-discordant cases were divided into 2 groups. One group (16.2% of cases) consisted of specimens with similar histology in curettage and hysterectomy, all belonging to the endometrioid subtype. This group showed DNA ploidy discordance because of a DNA diploid peak in 1 specimen and an aneuploid peak (DI=1.05-1.2) in the other. The other group (11.1% of cases) consisted of cases with different histologic subtype or grade and showed a more pronounced DNA ploidy difference (diploid vs. aneuploid with DI>1.2). Our results suggest that the DNA ploidy results of the hysterectomy and curettage specimens are not identical. The difference observed, which we believe reflects the intratumoral heterogeneity, should be taken into account when applying DNA ploidy to endometrial carcinoma specimens.
    International journal of gynecological pathology: official journal of the International Society of Gynecological Pathologists 09/2010; 29(6):572-8. DOI:10.1097/PGP.0b013e3181e2e8ee · 1.67 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: FIGO stage, tumor grade, depth of myometrial invasion, lymph-vascular space status and lymph node status are the most important clinical-pathological prognostic variables for endometrioid-type endometrial carcinoma. In the last years, several investigations have assessed different biological variables in tissue and serum samples from patients with this malignancy in order to detect biomarkers able to predict the clinical outcome. The present paper reviewed the literature data about the prognostic relevance of mutational status and/or immunohistochemical expression of p53, PTEN, PIK3, mTOR, β-catenin, k-ras and RASSF1A, microsatellite instability, vascular endothelial growth factor expression, DNA aneuploidy, and serum assay of CA125 and other tumor associated antigens. Tissue and serum biomarkers could be used, in addition to the conventional clinical-pathological variables, for the stratification of patients into categories with different risk of recurrence to better tailor adjuvant treatment.
    Critical reviews in oncology/hematology 12/2010; 80(2):181-92. DOI:10.1016/j.critrevonc.2010.11.005 · 4.03 Impact Factor
Show more