Antidiabetic potential of Rhodiola sachalinensis root extract in streptozotocin-induced diabetic rats.
ABSTRACT In this study, we examined the antidiabetic effect and probable mechanisms of Rhodiola sachalinensis root extract (RS). The extract was examined by thin-layer chromatographic analysis, and the main compound was determined to be a polysaccharide. In streptozotocininduced diabetic rats, RS showed significant hypoglycemic activity by lowering blood glucose (at doses of 200 and 400 mg/kg for 40 days). The levels of serum total cholesterol and triglycerides in RS-treated diabetic rats were lower than in control diabetic rats. A significant increase in the serum insulin levels of diabetic rats following RS treatment was also observed. Furthermore, RS treatment decreased malondialdehyde levels, while increasing superoxide dismutase, catalase and glutathione peroxidase activities of the liver and kidney of diabetic rats. At the same time, RS did not show any significant toxicity in LD(50) and single-cell gel electrophoresis assays. These results indicate that RS has hypoglycemic and hypolipidemic activities and is an effective scavenger of free radicals that inhibits lipid peroxidation. The antioxidant and pancreatic beta-cell-protective activities of RS may be the main mechanisms of the observed antidiabetic effect of RS.
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ABSTRACT: Based on the preliminary screening of eight indigenous putative probiotic Lactobacilli, Lactobacillus fermentum Lf1 was selected for assessing its antioxidative efficacy in DSS colitis mouse model based on its ability to enhance the expression of "Nrf2" by 6.43-fold and malondialdehyde (MDA) inhibition by 78.1 ± 0.24% in HT-29 cells under H2O2 stress. The Disease Activity Index and histological scores of Lf1-treated mice were lower than the control group. However, expression of "Nrf2" was not observed in Lf1-treated mice. A significant increase in the expression of antioxidative enzymes such as SOD2 and TrxR-1 was recorded in both of the groups. The expression of SOD2 was significantly downregulated in colitis-induced mice by -100.00-fold relative to control group, and the downregulation was considerably reduced to -37.04-fold in colitis Lf1 treatment group. Almost, a similar trend was recorded in case of "thioredoxin" expression, though "CAT" was refractile to expression. The Lf1-treated group had decreased malondialdehyde level as compared to colitis control (37.92 ± 6.31 versus 91.13 ± 5.76 μM/g). These results point towards Lf1-induced activation of the antioxidant enzyme system in the mouse model and its prospects to be explored as a new strategy for IBD management.BioMed Research International 01/2014; 2014:206732. DOI:10.1155/2014/206732 · 2.71 Impact Factor
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ABSTRACT: It has been confirmed that diabetes mellitus (DM) carries increased oxidative stress. This study evaluated the effects of salidroside from Rhodiolae Radix on diabetes-induced oxidative stress in mice. After induction of diabetes, diabetic mice were administered daily doses of 50, 100 and 200 mg/kg salidroside for 28 days. Body weights, fasting blood glucose (FBG), serum insulin, TC (total cholesterol), TG (triglyceride), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were measured. Results showed that salidroside possessed hypoglycemic activity and protective effects against diabetes-induced oxidative stress, which could significantly reduce FBG, TC, TG and MDA levels, and at same time increase serum insulin levels, SOD, GPx and CAT activities. Therefore, salidroside should be considered as a candidate for future studies on diabetes.Molecules 12/2011; 16(12):9912-24. DOI:10.3390/molecules16129912 · 2.42 Impact Factor
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ABSTRACT: Diabetes mellitus is a complicated metabolic disorder that has gravely troubled the human health and quality of life. Conventional agents are being used to control diabetes along with lifestyle management. However, they are not entirely effective and no one has ever been reported to have fully recovered from diabetes. Numerous medicinal plants have been used for the management of diabetes mellitus in various traditional systems of medicine worldwide as they are a great source of biological constituents and many of them are known to be effective against diabetes. Medicinal plants with antihyperglycemic activities are being more desired, owing to lesser side-effects and low cost. This review focuses on the various plants that have been reported to be effective in diabetes. A record of various medicinal plants with their established antidiabetic and other health benefits has been reported. These include Allium sativa, Eugenia jambolana, Panax ginseng, Gymnema sylvestre, Momrodica charantia, Ocimum sanctum, Phyllanthus amarus, Pterocarpus marsupium, Trigonella foenum graecum and Tinospora cordifolia. All of them have shown a certain degree of antidiabetic activity by different mechanisms of action.03/2012; 4(1):27-42. DOI:10.4103/0975-7406.92727