Évolution des techniques de séquençage

Technologies de Laboratoire 01/2007;
Source: DOAJ

ABSTRACT La biologie analytique a connu depuis quelques années une grande révolution en conséquence des progrès technologiques de la biologie moléculaire appliquée au séquençage des génomes. En effet, connaître l’enchaînement complet des bases nucléotidiques qui constituent un génome, c’est connaître toute l’information nécessaire à la vie (du moins en théorie).Ce n’est que récemment, avec la mise en place des Programmes Génome, que de nombreux génomes, dont celui de l’être humain, sont aujourd’hui séquencés, et que d’autres génomes sont en voie de l’être dans des délais de plus en plus raccourcis. Ces réalisations spectaculaires, sont rendues possible grâce aux développements extraordinaires des techniques de séquençage que nous essayons de passer en revue dans cet article. Nous rappellerons dans un premier temps le principe de base et l’évolution des techniques traditionnelles (Sanger, Maxam et pyroséquençage). Nous discuterons les avantages et les inconvénients de chaque approche avant d’exposer la nouvelle génération de séquenceurs automatisés.

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