High Inter-Laboratory Reproducibility of MALDI-TOF Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria.

A Mellmann, F Bimet, C Bizet, A. D. Borovskaya, R. R. Drake, U Eigner, A M Fahr, Y. He, E. N. Ilina, M Kostrzewa, T Maier, L Mancinelli, W. Moussaoui, G Prévost, L Putignani, C. L. Seachord, Y. W. Tang, D Harmsen

Institute for Hygiene, University Hospital Muenster, D-48149 Muenster, GERMANY; Centre de Ressources Biologiques de l'Institut Pasteur, Department of Microbiology, Institut Pasteur, F-75724 Paris, FRANCE; Research Institute of Physical-Chemical Medicine, 119992 Moscow, RUSSIA; Department of Microbiology and Molecular Cell Biology, Center for Biomedical Proteomics, Eastern Virginia Medical School, Norfolk, VA, USA; Labor Limbach, 69126 Heidelberg, GERMANY; Departments of Medicine and Pathology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA; Bruker Daltonik GmbH, Bremen, GERMANY; Microbiology Unit, Bambino Gesù Children's Hospital, Health Care and Research Institute, 00165 Rome, ITALY; Institut de Bactériologie UR Physiopathologie et Médicine Translationnelle, F-67000 Strasbourg, FRANCE; Department for Periodontology, University Hospital Muenster, D-48149 Muenster, GERMANY.

Journal Article: Journal of clinical microbiology (impact factor: 4.16). 09/2009; DOI: 10.1128/JCM.00921-09

Abstract

MALDI-TOF MS has emerged as rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (eight laboratories) achieved 98.75% inter-laboratory reproducibility. Only six of 480 samples were misidentified due to interchanges (4 samples) or contamination (1), or not identified because of insufficient signal intensity (1).

Source: PubMed

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Keywords

4 samples
 
cost-effective alternative
 
Identifying 60 blind-coded nonfermenting bacteria samples
 
insufficient signal intensity
 
international study