Analysis of essential oils of Artemisia absinthium L. from Lithuania by CC, GC(RI), GC-MS and 13C NMR.

Institute of Chemistry, Vilnius, Lithuania.
Natural product communications (Impact Factor: 0.96). 08/2009; 4(8):1113-8.
Source: PubMed

ABSTRACT Different techniques have been utilized to determine the composition of Artemisia absinthum (wormwood) essential oil. The oil was fractionated on a silica gel column and each fraction analyzed by GC(RI), GC-MS and 13C NMR. This allowed the identification, for the first time in A. absinthium, of two diterpenes, 9-geranyl-p-cymene and 9-geranyl-alpha-terpinene, and two homoditerpenes, 9-(15,16-dihydro-15-methylene)-geranyl-p-cymene and 9-(15,16-dihydro-15-methylene)-geranyl-alpha-terpinene. Chemical variability of A. absinthium essential oils from plants collected in the surroundings of Vilnius city over several years (1999-2007) was also shown. Chemical composition was determined by GC and GC-MS. Thujones (cis+trans, 10.2-36.3%) and trans-sabinyl acetate (9.8-39.2%) were the two predominant constituents of almost all the investigated oils (13 out of 15 samples). The third major compound was myrcene (5.1-9.2%, in four samples), beta-pinene (5.4-10.4%, in 5), linalool (4.7% in one), trans-sabinol (6.4%, in one) and 1,8-cineole (5.2-7.1%, in two). In one oil, the prevailing components were thujones (cis+trans, 11.2%), trans-sabinene hydrate (11.0%) and trans-sabinyl acetate (8.8%), while another sample was characterized by a large quantity of trans-sabinyl acetate (55.2%) and the absence of thujones.

  • [Show abstract] [Hide abstract]
    ABSTRACT: Nowadays, new strategies for pest and disease control to be used in rotation with or replacement of conventional pesticides are required. Essential oils (EOs), as botanical pesticides, provide a potential resource to develop more environmentally friendly and less toxic means of control to be applied in different produces. Tomato crop is affected by many insects and fungal diseases, among which, the insects Trialeurodes vaporariorum and Tuta absoluta, and the fungi Alternaria spp. and Botrytis cinerea are of great incidence. In this work two EOs from Uruguayan specimens of the local species Eupatorium buniifolium and the world-wide distributed Artemisia absinthium (Asteraceae) were characterized in their chemical composition and insecticidal and antifungal activities. We found that the EO from local A. absinthium is rich in oxygenated monoterpenes and belongs to the thujone chemotype (β-Thujone abundance is 56 ± 2 %, and α-Thujone, 1.67 ± 0.07 %). On the other hand, monoterpene hydrocarbons (α-Pinene, 22 ± 2 %) and sesquiterpene hydrocarbons [(E)-β-Guaiene, 10 ± 1 %] are the most abundant components of E. buniifolium EO. Eventhough both EOs chemically differ, they exhibit insecticidal and antifungal activity not only by direct contact but also by contact with their vapors against the tested organisms. These results may indicate that these EOs could be raw material to develop control agents to manage some of the main pests and fungal diseases of tomato crops with only one kind of treatment.
    Phytochemistry Reviews 01/2012; · 4.15 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The chemical composition of plant essential oils, and the in vitro effect of plant extracts and plant essential oils on the phytopathogens Bipolaris oryzae and Gerlachia oryzae was investigated. Liquid extracts of ten plants were obtained by maceration and then dialyzed using a 3kDa retention membrane. The plant essential oils from seven plants were analyzed by gas chromatography. To determine anti-fungal activity two methodologies were used: Kirby-Bauer and incubation. In this research we identified the major compounds of the essential oils: 93.77 % β-thujone in the Tanacetum vulgare oil; 40.61 % β-thujone in Artemisia absinthium; 45.89 % nananone and 41.07 % undecanone in Ruta graveolens and; 38.78 % citronellol and 19.55 % carvone in Malva sp. This research showed that the Cymbopogon citratus, P. alliacea, S. officinale and Malva sp. extracts have more potential to control G. oryzae best while the S. officinale and Lantana camara extracts are most efficient against B. oryzae. Among the plant essential oils, Zingiber officinale, C. citratus and Malva sp. had the greatest potential to control both phytopathogens. This is the first report of the activity of plant extracts and essential oils against the phytopathogens G. oryzae and B. oryzae, two important pathogens of irrigated rice.
    Australasian Plant Pathology 07/2013; 42(4):469-475. · 1.02 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: A large number of essential oils is reported to have significant activity against Candida albicans. But the different chemical composition influences the degree of their activity. The intention of this study was to investigate the chemical composition and the activity against Candida albicans of volatile oils obtained from Artemisia dracunculus, A. abrotanum, A. absinthium, and A. vulgaris (Asteraceae). The aim of the study was to identify new chemical compounds that have effect against C. albicans.The essential oils were obtained by hydrodistillation or extraction with dichloromethane (a new procedure we developed trying to obtain better, more separated compounds) from air dried above ground plant material and analyzed by GC-MS. Additionally commercial essential oils from the same species were tested. The Candida albicans inhibition studies were carried out by the paper disc diffusion method. The essential oils shared common components but presented differences in composition and showed variable antifungal activity. Davanone and derivatives thereof, compounds with silphiperfolane skeleton, estragole, davanone oil, beta-thujone, sabinyl acetate, herniarin, cis-chrysanthenyl acetate, 1,8-cineol, and terpineol were the main components of Artemisia volatiles. Among the volatile fractions tested those from A. abrotanum containing davanone or silphiperfolane derivatives showed the highest antifungal activity. The in vitro tests revealed that the Artemisia oils are promising candidates for further research to develop novel anti-candida drugs.
    Chemistry Central Journal 01/2014; 8(1):6. · 1.31 Impact Factor


Available from
May 22, 2014