Dam and its role in pathogenicity of Salmonella enterica

Centro de Estudios Farmacológicos y Botánicos CEFyBO-CONICET, Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
The Journal of Infection in Developing Countries (Impact Factor: 1.14). 08/2009; 3(7):484-90. DOI: 10.3855/jidc.465
Source: PubMed


Dam methylation is an essential factor involved in the virulence of an increasing number of bacterial pathogens including Salmonella enterica. Lack of Dam methylation causes severe attenuation in animal models. It has been proposed that dysregulation of Dam activity is potentially a general strategy for the generation of vaccines against bacterial pathogens. In this review, we focus our attention on the role of methylation by Dam protein in regulating bacterial gene expression and virulence in Salmonella enterica.

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    • "GipA has been shown to be a virulence gene in Salmonella enterica[32]. Analogously, DNA adenine methylase (Dam) is known as an important factor in bacterial virulence [43-45]. The above observations are consistent with the possibility that Fanzor protein could be a methytransferase. "
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    • "In the context of virulence, methylation has been shown to be an essential PTM in various bacterial pathogens. Using genetic approaches, native methylation has been shown to be required for regulation of transcription and phase variation of outermembrane components (Deitsch et al., 2009), and for maintenance of virulence (Giacomodonato et al., 2009). Methylation of glycolipids has been shown to be essential for Mycobacterium avium virulence in mice (Krzywinska et al., 2005), while methylation of proteins has been shown to alter the antigenicity of the outer-membrane protein OmpB from Rickettsia typhi (Chao et al., 2008), and to alter both the antigenicity of and the host T cell-mediated immune response against the heparin-binding haemagglutinin from Mycobacterium tuberculosis (Parra et al., 2004; Temmerman et al., 2004). "
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