Oxygen-ozone therapy in medicine: an update.
ABSTRACT Oxygen-ozone therapy, initially started as an empirical approach, has now reached a stage where most of the biological mechanisms of action of ozone have been clarified, showing that they are in the realm of orthodox biochemistry, physiology and pharmacology. Here we have reviewed a few relevant clinical applications and have shown that ozone therapy is particularly useful in cardiovascular disorders and tissue ischemia. In chronic viral infections, it is unable to eliminate the viremia but it may display supportive help by stimulating the immune system. Recently, its use has been successfully extended to the herniated disk pathology and therapy of primary caries in children.
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ABSTRACT: This study aimed to compare the antimicrobial efficacy of low temperature atmospheric pressure plasma (LTAPP) design and gaseous ozone delivery system with 2.5% NaOCl on E.faecalis in root canal walls and dentine tubules. The samples were divided into LTAPP (n=12), ozone (n=12), NaOCl (positive control, n=12); and saline (negative control, n=6) groups. Microbial samples were collected using paper points and dentin chips from root canals. Antimicrobial efficacy was assessed by counting the Colony Forming Units of E. faecalis before and after each irrigation protocol. Data were analyzed using Kruskall-Wallis, Wilcoxon signed-rank, Friedman and Bonferroni t (Dunn's test) tests (P=0.05). The microbial sampling with paper points showed antibacterial efficacy of NaOCl, LTAPP, ozone, and saline in descending order, respectively (P<0.05). The microbial sampling with dentin chips demonstrated a superior efficacy of LTAPP compared to NaOCl in the middle third (P<0.05), while both had similar effects in coronal and apical thirds (P>0.05). NaOCl and LTAPP were better than ozone at the coronal and middle parts of the root canals (P<0.05). These findings led us to suggest that LTAPP, which has no thermal and chemical effects, may be of great aid in endodontic treatment. This article is protected by copyright. All rights reserved.Letters in Applied Microbiology 08/2013; · 1.63 Impact Factor
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ABSTRACT: The aim of this study is to evaluate the effect of ozone and fluoride varnish on occlusal caries in primary molars in a split-mouth study. Caries risk was estimated by treating Public Dental Health Service dentists. Children with occlusal caries with Ekstrand index scores ≤3 (VI ≤3) were included. Selection of caries lesions was discontinued for ethical reasons due to non-acceptable clinical results during the follow-up. In the continued evaluation pairs of teeth with non-cavitated caries lesions, Ekstrand score ≤2a (VI ≤2) were selected. Fifty pairs of carious primary molars were included, 18 boys and 15 girls (mean 4.7 years, range 3-8). At baseline, the lesions were assessed by visual inspection (VI) and laser-induced fluorescence (LF), in each pair to treatment with 40 s ozone (HealOzone(TM), 2,100 ppm) or fluoride varnish Duraphat®. The treatments and evaluations were repeated at 3, 6 9 months and evaluations only at 12 months. Medium-high caries risk was observed in VI ≤3 children and low-medium risk in VI ≤2a children. In the 15 pairs VI ≤3 lesions, 8 treated with ozone and 9 with fluoride progressed to failure. In the 35 pairs VI ≤2a lesions, one lesion failed. Median baseline LF values in the VI ≤3 group were 76 and 69, for ozone and fluoride lesions, respectively, and 21 and 19 in the VI ≤2a group. At 12 months, LF values in the VI ≤2a group were 15 and 18. No improvement or difference in LF values was found over time between the caries lesions treated with ozone or fluoride. Neither ozone nor fluoride varnish treatments stopped the progression of caries in cavitated lesions. In low and medium caries risk children, non-cavitated lesions following both treatments showed slight or no progression. The use of ozone or fluoride varnish treatments in this regime as caries preventive method, added to the daily use of fluoridated toothpaste, to arrest caries progression in primary molars must therefore be questioned.Clinical Oral Investigations 12/2013; · 2.20 Impact Factor
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ABSTRACT: The objective of this study was to evaluate the interaction between ozone oxidative preconditioning and the anesthetic ketamine on cytoplasmic nuclear factor κB (NF-κB) levels in a rat endotoxic shock model. Forty Wistar rats were randomly divided into 5 groups: I - control group; II - rats intraperitoneally (i.p.) treated with LPS; III - rats treated with LPS and then treated with ketamine; IV - animals pre-treated with O(3)/O(2) mixture for 5 days, then treated with LPS for 24 h followed by infusion with ketamine; V - O(3)/O(2) pre-treatment, as described above for IV, followed by LPS infusion, and then 0.9% saline. In addition to histological examination of the liver, the levels of NF-κB were determined by SABC immunohistochemistry in each group. Histological damage was observed in the lipopolysaccharide (LPS) group. It was characterized by hepatic disarray, hepatic lobule distortion, congestion of liver sinusoids, hepatocyte swelling and necrosis, and granulocyte infiltration. These changes were not obvious in the O(3)/O(2) + LPS + ketamine group. The normal control group had low activity of NF-κB, but that activity was markedly increased in the LPS group (p < 0.05). The NF-κB level was significantly decreased in the O(3)/O(2) + LPS + ketamine group (p < 0.05) when compared with the ketamine-treated group, and was almost equal to the control group. We confirmed that the preconditioning effect of ozone enhances the biological effectiveness of ketamine by altering NF-κB activity, which may play an important role in sepsis-induced liver injury in rats.Archives of medical science : AMS. 11/2012; 8(5):918-23.