Oxygen-ozone therapy in medicine: an update.
ABSTRACT Oxygen-ozone therapy, initially started as an empirical approach, has now reached a stage where most of the biological mechanisms of action of ozone have been clarified, showing that they are in the realm of orthodox biochemistry, physiology and pharmacology. Here we have reviewed a few relevant clinical applications and have shown that ozone therapy is particularly useful in cardiovascular disorders and tissue ischemia. In chronic viral infections, it is unable to eliminate the viremia but it may display supportive help by stimulating the immune system. Recently, its use has been successfully extended to the herniated disk pathology and therapy of primary caries in children.
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ABSTRACT: The objective of this study was to evaluate the interaction between ozone oxidative preconditioning and the anesthetic ketamine on cytoplasmic nuclear factor κB (NF-κB) levels in a rat endotoxic shock model. Forty Wistar rats were randomly divided into 5 groups: I - control group; II - rats intraperitoneally (i.p.) treated with LPS; III - rats treated with LPS and then treated with ketamine; IV - animals pre-treated with O(3)/O(2) mixture for 5 days, then treated with LPS for 24 h followed by infusion with ketamine; V - O(3)/O(2) pre-treatment, as described above for IV, followed by LPS infusion, and then 0.9% saline. In addition to histological examination of the liver, the levels of NF-κB were determined by SABC immunohistochemistry in each group. Histological damage was observed in the lipopolysaccharide (LPS) group. It was characterized by hepatic disarray, hepatic lobule distortion, congestion of liver sinusoids, hepatocyte swelling and necrosis, and granulocyte infiltration. These changes were not obvious in the O(3)/O(2) + LPS + ketamine group. The normal control group had low activity of NF-κB, but that activity was markedly increased in the LPS group (p < 0.05). The NF-κB level was significantly decreased in the O(3)/O(2) + LPS + ketamine group (p < 0.05) when compared with the ketamine-treated group, and was almost equal to the control group. We confirmed that the preconditioning effect of ozone enhances the biological effectiveness of ketamine by altering NF-κB activity, which may play an important role in sepsis-induced liver injury in rats.Archives of medical science : AMS. 11/2012; 8(5):918-23.
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ABSTRACT: This study aimed to compare the antimicrobial efficacy of low temperature atmospheric pressure plasma (LTAPP) design and gaseous ozone delivery system with 2.5% NaOCl on E.faecalis in root canal walls and dentine tubules. The samples were divided into LTAPP (n=12), ozone (n=12), NaOCl (positive control, n=12); and saline (negative control, n=6) groups. Microbial samples were collected using paper points and dentin chips from root canals. Antimicrobial efficacy was assessed by counting the Colony Forming Units of E. faecalis before and after each irrigation protocol. Data were analyzed using Kruskall-Wallis, Wilcoxon signed-rank, Friedman and Bonferroni t (Dunn's test) tests (P=0.05). The microbial sampling with paper points showed antibacterial efficacy of NaOCl, LTAPP, ozone, and saline in descending order, respectively (P<0.05). The microbial sampling with dentin chips demonstrated a superior efficacy of LTAPP compared to NaOCl in the middle third (P<0.05), while both had similar effects in coronal and apical thirds (P>0.05). NaOCl and LTAPP were better than ozone at the coronal and middle parts of the root canals (P<0.05). These findings led us to suggest that LTAPP, which has no thermal and chemical effects, may be of great aid in endodontic treatment. This article is protected by copyright. All rights reserved.Letters in Applied Microbiology 08/2013; · 1.63 Impact Factor
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ABSTRACT: Abstract Objectives: The objectives of this study were to evaluate the effectiveness and tolerability of ozone therapy by rectal insufflation as add-on therapy in fibromyalgia management. Design: Patients with fibromyalgia received 24 sessions of ozone therapy during a 12-week period. At each session, the administered dose of ozone was 8 mg (200 mL of gas, at a concentration of 40 μg/mL). Ozone sessions were given 5 days a week during the first 2 weeks, twice a week from weeks 3-6, and weekly from weeks 7-12. Fibromyalgia Impact Questionnaire (FIQ) was the main outcome measure, and was administered at baseline and at weeks 4, 8, and 12. Secondary outcome measures, administered at baseline and at endpoint, were the Pittsburgh Sleep Quality Index, the Beck Depression Inventory, the State and Trait Anxiety Inventory, and the SF-12, the abbreviated form of the Short Form Health Survey. Emergent adverse reactions to treatment were recorded. Results: FIQ total scores decreased significantly during the study period, with the decrease being observed in the first 4 weeks of the study. Significant improvement was also seen both in depression scores and in the Physical Summary Score of the SF-12. Transient meteorism after ozone therapy sessions was the most frequently reported side-effect. Conclusions: At the dose and number of sessions used in this study, ozone therapy by rectal insufflation seems to be beneficial for physical symptoms and depression of fibromyalgia.Journal of alternative and complementary medicine (New York, N.Y.) 10/2012; · 1.69 Impact Factor