Gold nanoparticle arrangement on viral particles through carbohydrate recognition: a non-cross-linking approach to optical virus detection.
ABSTRACT We propose a new approach to optical virus detection based on the spatial assembly of gold nanoparticles on the surface of viruses. Since JC virus-like particles (VLPs) comprise a repeating viral capsid protein that binds to sialic acid, the conjugation of sialic acid-linked Au particles with VLPs enables the spatial arrangement of Au particles on the VLP surface. This structure produced a red shift in the absorption spectrum due to plasmon coupling between adjacent Au particles, leading to the construction of an optical virus detection system. Our system depends not on the simple cross-linking of VLPs and Au particles, but on an ordered Au structure covering the entire surface of the VLPs and can be applied to various virus detection systems using the inherent ligand recognition of animal viruses.
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ABSTRACT: One promising, but currently underexplored, area for the future of drinking water pathogen monitoring stems from the development of nanomaterial-enabled detection strategies. The nanoscience literature contains numerous reports of nanoenabled biosensors; however, to date only a small percentage have focused on the detection of whole cells, in general, and waterborne pathogens, in particular. There are significant opportunities for the use of nanoenabled biosensors for environmental monitoring, and this review is intended to both illustrate the state of this field and to spur additional research in this area.Environmental Science and Technology 05/2010; 44(10):3656-69. · 5.23 Impact Factor