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Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines.

Celal Bayar University, Faculty of Medicine, Department of Histology and Embryology, 35040 Bornova, Izmir, Manisa, Turkey.
Acta histochemica (Impact Factor: 1.76). 10/2009; 112(6):546-56. DOI: 10.1016/j.acthis.2009.06.001
Source: PubMed

ABSTRACT Propolis is a sticky substance that is collected from plants by honeybees that has anti-mutagenic and anti-carcinogenic properties with biological and therapeutic effects. The target of this study was to investigate the anti-apoptotic effect of propolis extracts (PE) on the caspase pathway in the human breast cell line MCF-7 in culture. Seven different propolis extracts, numbered PE 1-7, produced in their natural ecological environment, were collected from the Hacettepe University Beytepe Campus area in Ankara, Turkey. Individual extracts at 0.5, 0.25, 0.125 and 0.063mg/ml were incubated with MCF-7 cells during 2 days culture. Cell growth and cytotoxicity were measured colorimetrically by MTT assay. Apoptotic cell death was determined by the TUNEL method (terminal deoxynucleotidyltransferase-biotin nick end-labelling) and caspase activity was investigated by immunocytochemistry using antibodies directed against caspase 6, caspase 8 and caspase 9. The results showed that the PE 5 and 6 extracts at 0.125mg/ml dilution induced apoptosis in association with increased number of TUNEL positive cells. MTT results showed that cultures exposed to the same extracts and at the same dilution experienced better cell growth compared to those cultures exposed to the other extracts. Immunpositivity for all caspases was detected after treatment with all the extracts and at all dilutions, with stronger immunoreactivity for caspase 6 than caspases 8 and 9. Caspase 6 labelling was especially strong in PE 5 and PE 6. We conclude that propolis may have anti-tumour effects by increasing apoptosis through the caspase pathway. Such propolis extracts may be important economically and allow development of a relatively inexpensive cancer treatment.

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    • "Propolis also known as bee glue is a dark resinous substance collected by the worker bees from various plant exudates which they use to fill spaces and to coat walls of the hive for protection [1] [2]. It has a long history of medicinal use by humans [3] and has captured the attention of many researchers as a result of its antiviral, antifungal, antibacterial, anti-inflammatory, antioxidant, and anticarcinogenic properties [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20]. "
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    ABSTRACT: Propolis (a bee product) which has a long history of medicinal use by humans has attracted a great deal of research interest in the recent time; this is due to its widely reported biological activities such as antiviral, antifungal, antibacterial, anti-inflammatory, antioxidant, and anticarcinogenic properties. Crude form of propolis and its phenolic contents have both been reported to exhibit antileukaemic effects in various leukaemia cell lines. The ability of the polyphenols found in propolis to arrest cell cycle and induce apoptosis and differentiation in addition to inhibition of cell growth and proliferation makes them promising antileukaemic agents, and hence, they are believed to be a key to the antileukaemic effects of propolis in different types of leukaemia. This paper reviews the molecular bases of antileukaemic activity of both crude propolis and individual polyphenols on various leukaemia cell lines, and it indicates that propolis has the potential to be used in both treatment and prevention of leukaemia. This however needs further evaluation by in vitro, in vivo, and epidemiological studies as well as clinical trials.
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    • "It has several biological properties as antimicrobial, antiinflammatory, immunomodulatory , and antioxidant [4] [5] [6]. It have been used since ancient times as a medicine because of its biological properties as antiinflammatory, antiallergic properties, anticarcinogenic , antioxidative, antifungal, antiviral, immunostimulant , and for tissue regeneration [7] [8] [9]. Administration of propolis alleviated the harmful effects of insecticide, propetamphos [10]. "
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