Article

Heat stress activates interleukin-8 and the antioxidant system via Nrf2 pathways in human dental pulp cells.

Department of Conservative Dentistry, The Institute of Oral Health Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
Journal of endodontics (impact factor: 2.95). 10/2009; 35(9):1222-8. DOI:10.1016/j.joen.2009.06.005 pp.1222-8
Source: PubMed

ABSTRACT This study tested whether heat stress (42 degrees C for 30 minutes) induces reactive oxygen species (ROS), proinflammatory cytokines, Nrf2 activation, and Nrf2 target genes such as antioxidant enzymes in human dental pulp (HDP) cells.
ROS was evaluated by using flow cytometry. Proteins and messenger RNA levels for cytokines and antioxidant genes were determined by using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR) analysis, respectively.
Heat stress induced the production of ROS and the increased expression of the interleukin (IL)-8 and IL-8 receptor genes. Exposure of cells to heat stress resulted in the nuclear translocation of Nrf2 and increased expression of Nrf2 target genes including heme oxygenase-1. Pretreatment with an exogenous antioxidant inhibited the heat-induced expression of IL-8 and Nrf2 target genes and Nrf2 translocation.
Collectively, these results show that heat-induced Nrf2 activation is the major regulatory pathway of cytoprotective gene expression against oxidative stress in HDP cells.

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Keywords

30 minutes
 
42 degrees C
 
antioxidant enzymes
 
antioxidant genes
 
cytokines
 
exogenous antioxidant inhibited
 
flow cytometry
 
heme oxygenase-1
 
human dental pulp
 
IL-8 receptor genes
 
interleukin
 
major regulatory pathway
 
messenger RNA levels
 
Nrf2 target genes
 
nuclear translocation
 
oxidative stress
 
Pretreatment
 
proinflammatory cytokines
 
reverse transcription-polymerase chain reaction
 
Western blotting