An M-CSF Receptor c-Fms Antibody Inhibits Mechanical Stress-Induced Root Resorption during Orthodontic Tooth Movement in Mice
ABSTRACT To examine the effect of anti-c-Fms antibody on odontoclastogenesis and root resorption in an orthodontic tooth movement mouse model.
We used orthodontic tooth movement in which an Ni-Ti coil spring was inserted between the upper incisors and the upper first molar. Root resorption occurred in this model. Anti-c-Fms antibody was injected daily into a local site for 12 days during mechanical loading. Odontoclastogenesis and root resorption were assessed by histology and scanning electron microscopy.
The anti-c-Fms antibody significantly inhibited odontoclastogenesis and root resorption during orthodontic tooth movement.
M-CSF and/or its receptor is a potential therapeutic target in mechanical stress- induced odontoclastogenesis, and injection of an anti-c-Fms antibody might be useful for inhibition of mechanical stress-induced root resorption during orthodontic tooth movement.
SourceAvailable from: Hideki Kitaura[Show abstract] [Hide abstract]
ABSTRACT: Mechanical force loading exerts important effects on the skeleton by controlling bone mass and strength. Several in vivo experimental models evaluating the effects of mechanical loading on bone metabolism have been reported. Orthodontic tooth movement is a useful model for understanding the mechanism of bone remodeling induced by mechanical loading. In a mouse model of orthodontic tooth movement, TNF- α was expressed and osteoclasts appeared on the compressed side of the periodontal ligament. In TNF-receptor-deficient mice, there was less tooth movement and osteoclast numbers were lower than in wild-type mice. These results suggest that osteoclast formation and bone resorption caused by loading forces on the periodontal ligament depend on TNF- α . Several cytokines are expressed in the periodontal ligament during orthodontic tooth movement. Studies have found that inflammatory cytokines such as IL-12 and IFN- γ strongly inhibit osteoclast formation and tooth movement. Blocking macrophage colony-stimulating factor by using anti-c-Fms antibody also inhibited osteoclast formation and tooth movement. In this review we describe and discuss the effect of cytokines in the periodontal ligament on osteoclast formation and bone resorption during mechanical force loading.The Scientific World Journal 01/2014; 2014:617032. DOI:10.1155/2014/617032 · 1.73 Impact Factor
Conference Paper: Light-Emitting Diode (LED) Therapy On Root Resorption In Rats[Show abstract] [Hide abstract]
ABSTRACT: Objectives: to analyze the effects of LED therapy at 940 nm on inflammatory cell infiltration and root resorption in an experimental model of orthodontic tooth movement in rats. Methods: Twenty-five male Wistar rats were allocated in four experimental groups: Control animals (Co, n=5), Controls + LED therapy (CoLED, n=6), animals submitted to orthodontic force (RR, n=7) and submitted to orthodontic force + LED therapy (RRLED, n=7). A force of 50g was applied to the right upper molars of RR and RRLED groups. On day 2, 3 and 4 after orthodontic appliance, CoLED and RRLED groups received LED irradiation (940nm, 4J/cm2). The animals were sacrificed at day 7 for histological analysis. Results: increased number of root resorption lacunae was found in RR group ( 7.5 ± 2.1 lacunae/105 µm root length, p < 0.05) in relation RRLED (0.5 ± 0.3). RR group also presented increased number of osteoclasts (1.17 ± 0.18 cells/105 µm root length, p < 0.005) and inflammatory cell infiltration (1.23 ± 0.8 cells/100 µm2 periodontal ligament, p < 0.005) in relation to RRLED group (0.12 ± 0.04 and 0.23 ± 0.3, respectively). Co and CoLED groups did not present resorptions lacunae and inflammatory cell infiltrate. Conclusion: The results suggest that LED therapy decreased inflammation and root resorption induced by orthodontic force.IADR General Session 2012; 06/2012
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ABSTRACT: Summary OBJECTIVES : Interleukin-4 (IL-4) is a recognized immunomodulatory cytokine that regulates bone homeostasis. However, the influence of IL-4 on orthodontic tooth movement (OTM) and subsequent root resorption is still unknown. Therefore, the purpose of this study was to investigate the effect of IL-4 on tooth movement and its associated root resorption in a mouse model.The European Journal of Orthodontics 07/2014; 37(1). DOI:10.1093/ejo/cju016 · 1.39 Impact Factor