A new effective scaffold to facilitate peripheral nerve regeneration: Chitosan tube coated with maggot homogenate product
ABSTRACT Recent efforts in scientific research in the field of peripheral nerve regeneration have been directed towards the development of artificial nerve guides. Chitosan tubes applied as a biocompatible and biodegradable bilateral guide for nerve repair is a hot spot in research to date. In previous study, we have found the homogenate product from disinfected maggot larvae can promote wound nerve regeneration and neuropeptides release. Wound nerves belong to the peripheral nerve system. We thus hypothesize that maggot homogenate product use as an external layer outside the chitosan tube will be an effective therapy to facilitate peripheral nerve regeneration.
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ABSTRACT: MEDICINAL MAGGOTS ARE BELIEVED TO HAVE THREE MAJOR MECHANISMS OF ACTION ON WOUNDS, BROUGHT ABOUT CHEMICALLY AND THROUGH PHYSICAL CONTACT: debridement (cleaning of debris), disinfection, and hastened wound healing. Until recently, most of the evidence for these claims was anecdotal; but the past 25 years have seen an increase in the use and study of maggot therapy. Controlled clinical studies are now available, along with laboratory investigations that examine the interaction of maggot and host on a cellular and molecular level. This review was undertaken to extract the salient data, make sense, where possible, of seemingly conflicting evidence, and reexamine our paradigm for maggot-induced wound healing. Clinical and laboratory data strongly support claims of effective and efficient debridement. Clinical evidence for hastened wound healing is meager, but laboratory studies and some small, replicated clinical studies strongly suggest that maggots do promote tissue growth and wound healing, though it is likely only during and shortly after the period when they are present on the wound. The best way to evaluate-and indeed realize-maggot-induced wound healing may be to use medicinal maggots as a "maintenance debridement" modality, applying them beyond the point of gross debridement.Evidence-based Complementary and Alternative Medicine 03/2014; 2014:592419. DOI:10.1155/2014/592419 · 2.18 Impact Factor
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ABSTRACT: The transdifferentiation of human adipose-derived stem cells (ADSCs) into Schwann-like cells on biocomposite scaffolds may be a critical issue in nerve regeneration medicine. In this study, tissue-engineered scaffold with chitosan nano powders (CS) and poly (lactide-co-glycolide) (PLGA) was investigated for its potential Schwann cells (SCs) transdifferentiation. The differentiation of human ADSCs into S-like cells was induced with different CS content and direction of nanofibers on PLGA/CS scaffolds. Cell morphology and proliferation of differentiated cells were investigated by scanning electron microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay respectively. For assessment efficiency of transdifferentiation, the expression of SC markers (glial fibrillary acidic protein and S100), and myelinogenic marker (myelin basic protein) were investigated in different nanochitosan content and direction of nanofibers scaffolds, using immunocytochemistry technique. The nanochitosan can significantly promote cell proliferation of differentiated cells (p< 0.05). The mean percentage of S-like cells on greater CS content nanofibers scaffold was significantly higher than others (p< 0.05). In addition, the align orientation of nanofibers in scaffolds guided the differentiation of ADSCs toward myelinating S-like cells on the constructs. Overall, we found that high CS content and aligned-orientation of nanofibers in biocomposite scaffold (70/30A) can promote differentiation and myelinogenic capacity of S-like cells induced from human ADSCs. This article is protected by copyright. All rights reserved. © 2015 Wiley Periodicals, Inc.Journal of Biomedical Materials Research Part A 01/2015; DOI:10.1002/jbm.a.35398 · 2.83 Impact Factor
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ABSTRACT: Polyhydroxyalkanoates (PHAs) have been demonstrated to be a family of biopolymers with good biodegradability and biocompatibility. Designed scaffolds comprising natural and synthetic materials are now widely used in the reconstruction of damaged tissues especially neural tissues. Polyhydroxyalkanoate scaffolds with properties similar to neural structures can be more effective in the reconstruction process. Better cell adhesion and migration, more guiding of axons, and structural features, such as porosity, provide a clearer role in the restoration of neural tissues. Here application of polyhydroxyalkonates as the potential biomaterials in improving performance of injured nerves and neural reconstruction is reviewed.International Journal of Polymeric Materials 11/2014; 63(17). DOI:10.1080/00914037.2014.886227 · 2.78 Impact Factor