Article

The development of genipin-crosslinked poly(caprolactone) (PCL)/gelatin nanofibers for tissue engineering applications.

Department of Biomedical Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Korea.
Macromolecular Bioscience (impact factor: 3.89). 09/2009; 10(1):91-100. DOI:10.1002/mabi.200900168 pp.91-100
Source: PubMed

ABSTRACT Composite nanofibers of poly(caprolactone) (PCL) and gelatin crosslinked with genipin are prepared. The contact angles and mechanical properties of crosslinked PCL-gelatin nanofibers decrease as the gelatin content increases. The proliferation of myoblasts is higher in the crosslinked PCL-gelatin nanofibers than in the PCL nanofibers, and the formation of myotubes is only observed on the crosslinked PCL-gelatin nanofibers. The expression level of myogenin, myosin heavy chain, and troponin T genes is increased as the gelatin content is increased. The results suggest that PCL-gelatin nanofibers crosslinked with genipin can be used as a substrate to modulate proliferation and differentiation of myoblasts, presenting potential applications in muscle tissue engineering.

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Keywords

Composite nanofibers
 
contact angles
 
crosslinked PCL-gelatin nanofibers
 
crosslinked PCL-gelatin nanofibers decrease
 
expression level
 
gelatin crosslinked
 
muscle tissue engineering
 
myosin heavy chain
 
myotubes
 
PCL nanofibers
 
PCL-gelatin nanofibers crosslinked
 
potential applications
 
substrate