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Mesenchymal stem cells and inorganic bovine bone mineral in sinus augmentation: comparison with augmentation by autologous bone in adult sheep

Universitätsklinik für Zahn-, Mund- und Kieferheilkunde, Abteilung Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Hugstetter Str. 55, D-79106 Freiburg, Germany.
British Journal of Oral and Maxillofacial Surgery (Impact Factor: 1.13). 09/2009; 48(4):285-90. DOI: 10.1016/j.bjoms.2009.06.226
Source: PubMed

ABSTRACT Our aim was to compare the osteogenic potential of mononuclear cells harvested from the iliac crest combined with bovine bone mineral (BBM) (experimental group) with that of autogenous cancellous bone alone (control group). We studied bilateral augmentations of the sinus floor in 6 adult sheep. BBM and mononuclear cells (MNC) were mixed and placed into one side and autogenous bone in the other side. Animals were killed after 8 and 16 weeks. Sites of augmentation were analysed radiographically and histologically. The mean (SD) augmentation volume was 3.0 (1.0) cm(3) and 2.7 (0.3) cm(3) after 8 and 16 weeks in the test group, and 2.8 (0.3) cm(3) (8 weeks) and 2.8 (1.2) cm(3) (16 weeks) in the control group, respectively. After 8 weeks, histomorphometric analysis showed 24 (3)% BBM, and 19 (11)% of newly formed bone in the test group. The control group had 20 (13%) of newly formed bone. Specimens after 16 weeks showed 29 (12%) of newly formed bone and 19 (3%) BBM in the test group. The amount of newly formed bone in the control group was 16 (6%). The results show that mononuclear cells, including mesenchymal stem cells, in combination with BBM as the biomaterial, have the potential to form bone.

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Available from: Michele Maglione, Dec 15, 2013
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    • "Split mouth study in 18 adult japanese rabbits Gutwald et al. (32) 2010 MSC: 19% (8 weeks), 29% (16 weeks) particulated autograft: 20% (8 weeks), 16% (16 weeks) MSC combined with bovine bone mineral have the potential to form bone Case series human study Shayesteh et al. (33) 2008 41% of regenerated bone (3 months) Less than 3 mm of residual bone. 6 patients. "
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    • "The addition of platelet-rich plasma which contains various growth factors on DBB did not enhance early and late healing of the bone [13]. The local delivery of mesenchymal stem cells (MSC) by DBB offers the promising potential of augmenting the healing of CSBD [14] [15], but it needs harvesting a cell from a secondary site, which is then expanded in vitro Bone 56 (2013) 110–118 ⁎ Corresponding author. Fax: +31 20 598 0333. "
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