Nocardioides panacisoli sp. nov., isolated from the soil of a ginseng field.
ABSTRACT A Gram-positive, rod-shaped, non-spore-forming bacterium (Gsoil 346(T)) was isolated from the soil of a ginseng field in South Korea and was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequences, strain Gsoil 346(T) was shown to belong to the genus Nocardioides in the family Nocardioidaceae, with the most closely related species being Nocardioides aquiterrae GW-9(T) (96.6 % 16S rRNA gene sequence similarity); however, the strain clustered in a distinct branch of the phylogenetic tree with Nocardioides kongjuensis A2-4(T) (96.2 %), Nocardioides aromaticivorans H-1(T) (96.1 %), Nocardioides nitrophenolicus NSP41(T) (96.1 %) and Nocardioides simplex ATCC 15799(T) (95.9 %). Strain Gsoil 346(T) was characterized chemotaxonomically and found to have ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, phosphatidylinositol and phosphatidylglycerol as the major polar lipids, MK-8(H(4)) as the predominant menaquinone and iso-C(16 : 0), C(18 : 1)omega9c and C(17 : 1)omega8c as the major fatty acids. The G+C content of the genomic DNA of the novel strain was 73.0 mol%. These chemotaxonomic properties supported the placement of strain Gsoil 346(T) in the genus Nocardioides. The results of physiological and biochemical tests, along with the phylogenetic analysis, allowed strain Gsoil 346(T) to be differentiated genotypically and phenotypically from recognized species of the genus Nocardioides. Therefore, strain Gsoil 346(T) represents a novel species, for which the name Nocardioides panacisoli sp. nov. is proposed, with Gsoil 346(T) (=KCTC 19470(T)=DSM 21348(T)) as the type strain.
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ABSTRACT: A gram-positive, non-motile, rod-shaped, psychrophilic actinomycete, designated strain Cr7-14(T), was isolated from alpine glacier cryoconite. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cr7-14(T) was related to members of the genus Nocardioides and shared highest 16S rRNA gene sequence similarities with the type strains of Nocardioides furvisabuli (98.6 %), Nocardioides ganghwensis (98.2 %), Nocardioides oleivorans (98.1 %) and Nocardioides exalbidus (97.6 %). The predominant cellular fatty acids of strain Cr7-14(T) were C(17 : 1)ω8c (39.5 %) and iso-C(16 : 0) (32.4 %). The major menaquinone was MK-8(H(4)). The diagnostic diamino acid in the cell-wall peptidoglycan was ll-2,6-diaminopimelic acid. The predominant cell-wall sugars were galactose and rhamnose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, four unknown glycolipids and two unknown polar lipids. The genomic DNA G+C content was 71.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species, Nocardioides alpinus sp. nov., is proposed, with Cr7-14(T) ( = DSM 23325(T) = LMG 26053(T) = CGMCC 1.10697(T)) as the type strain.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 62(Pt 2):445-50. · 2.11 Impact Factor
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ABSTRACT: A Gram-reaction-positive, rod-shaped, non-motile, non-spore-forming bacterium (strain BX5-10(T)) was isolated from the soil of a ginseng field on Baekdu Mountain in Jilin district, China. The taxonomic position of this bacterium was determined in an investigation based on a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain BX5-10(T) was shown to belong to the family Nocardioidaceae and to be most closely related to Nocardioides plantarum NCIMB 12834(T) (96.5% sequence similarity), Nocardioides dokdonensis KCTC 19309(T) (96.2%) and Nocardioides fonticola NAA-13(T) (95.1%). Strain BX5-10(T) was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in its cell-wall peptidoglycan, MK-8(H(4)) as the predominant menaquinone and C(18:1)ω9c, C(16:0) and C(17:1)ω8c as its major fatty acids. The G+C content of the genomic DNA was 70.3 mol%. The novel strain could be differentiated genotypically and phenotypically from all recognized species of the genus Nocardioides. Based on the results of the phylogenetic analyses and the genotypic and phenotypic data, a novel species, Nocardioides ginsengagri sp. nov., is proposed. The type strain is BX5-10(T) (=KCTC 19467(T)=DSM 21362(T)).INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 04/2011; 62(Pt 3):591-5. · 2.11 Impact Factor